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Catalog of available lenti-viral vectors

Here you will find an overview of our various lentiviral plasmid vectors. You can enter the corresponding catalog number in our order system and have your desired virus produced.

External users must complete an MTA between the Charité and its facility. For some vectors, corresponding Addgene regulations must be applied. You will receive further information during the ordering process

 

 

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Lenti-viral tools for your research

Which vector can we help you with? Sorted by their specific transgenes or functions, you will find various tools that could help you in your scientific research.

We try to keep the list up to date. Please contact us if your desired vector is not included. Use our DNA service if we could modify existing vectors specifically for you.

Calcium sensors

Catalog #

Vector name

Promoter

Gene of Interest

Discription

MTA informations

Ref for publication

BL-0700

f(syn)-Syp-GCamp6f-w

hSynapsin1

Synaptophysin,GCamp6f

Synaptophysin fused C terminally with GCamp6f (subclone of addgene #40755)

Addgene #40755

Chen et al. 2013

BL-0570

f(syn)-NLS.GFP-P2A-GCamp6f-w

hSynapsin1

GCamp6f

Nuclear GFP linked via P2A to GCamp6f (Subclone of addgene #40755)

Addgene #40755

Chen et al. 2013

BL-1231

f(syn)-NLS.CFP-P2A-Syp-GCamp6f

hSynapsin1

Synaptophysin, GCamP6f, NLS.CFP

Nuclear CFP linked via P2A to GCamp6f (Subclone of addgene #40755)

Addgene #40755

Chen et al. 2013

BL-1331

f(syn)SWITCH(Syp-GCamp6f)

hSynapsin1

Synaptophysin,GCamp6f

Cre-ON: Synaptophysin fused C terminally with GCamp6f (subclone of addgene #40755)

Addgene #40755

Chen et al. 2013

BL-1338

f(syn)-Flox(Syp-GCamp6f)

hSynapsin1

Synaptophysin,GCamp6f

Cre-OFF: Synaptophysin fused C terminally with GCamp6f (subclone of addgene #40755)

Addgene #40755

Chen et al. 2013

BL-1329

f(syn)-Syp-jRCaMP1b

hSynapsin1

Synaptophysin, jRCaMP1b

Synaptophysin fused C terminally with jRCaMP1b (subclone of addgene #63136)

Addgene #63136

Dana et al. 2016

BL-1505

f(syn)-NES-jRCaMP1b-WPRE

hSynapsin1

NES-jRCaMP1b

Subclone of addgene #63136

Addgene #63136

Dana et al. 2016

BL-1471

pLEX-R_GECO1

hPGK

R-GECO1

Expression of R-GECO1.0 under the control of the hPGK Promoter. Subclone of Addgene #32444 in pLex_305 (Addgene #41390)

Addgene #32444 and Addgene #41390

Zhao et al. 2011

pH sensors

Catalog #

Vector name

Promoter

Gene of Interest

Discription

MTA informations

Ref for publication

BL-0047

f(syn)-SypHy-w

hSynapsin1

Synaptophysin, pHluorin

Expression of Rat Synaptophysin-pHluorin, subclone of addgene #24478

Addgene #24478

Granseth et al. 2006

BL-0099

f(syn)-Syb2-pH

hSynapsin1

Synaptpbrevin2 (Vamp2), pHluorin

Synaptobrevin2 (Vamp2) fused c-termially to pHluorin, subclone of addgene #24478

Addgene #24478

Granseth et al. 2006

BL-0664

f(syn)Switch(revSyphy)

hSynapsin1

Synaptophysin, pHluorin

Cre-On expression of Rat Synaptophysin-pHluorin, subclone of addgene #24478

Addgene #24478

Herman et al. 2018

BL-0676

f(syn)Flox(Syphy)

hSynapsin1

Synaptophysin, pHluorin

Cre-off: expression of Rat Synaptophysin-pHluorin in non-Cre expressing cells, subclone of addgene #24478

Addgene #24478

Herman et al. 2018

BL-0647

f(syn)SWITCH(RFP_revSypHy)w

hSynapsin1

Synaptophysin, pHluorin

CreON/OFF Vector: Expression of RFP in Non-Cre cells, and of Rat Synaptophysin-pHluorin in Cre cells. Subclone of addgene #24478

Addgene #24478

Granseth et al. 2006

HL-0121

f(syn)-VGlut1pH-w

hSynapsin1

VGlut1 , pHluorin

VGlut1 fused to pHLuorin in within the luminal part of the Transporter

-

Herman et al. 2014

BL-0247

f(syn)-VGlut2pH-w

hSynapsin1

VGlut2 , pHluorin

VGlut2 fused to pHLuorin in within the luminal part of the Transporter

-

Herman et al. 2014

BL-0336

f(syn)-Phönix-w

hSynapsin1

Halorhodopsin, SypHy and mkate2

Halorhodopsin fused to SypHy and mkate2 (synaptic vesicle proton pump and pH sensor)

Addgene #70111

Rost et al. 2016

BL-0344

f(syn)-Syt1-pHluorin-w

hSynapsin1

Synaptotagmin1, pHluorin

Synaptotagmin1 fused N terminally with pHLuorin, subclone of addgene #24478

Addgene #24478

-

BL-0553

f(syn)-Syp-pH-mkate2-w

hSynapsin1

Synaptophysin, luminal pHluorin

Synaptophysin with Luminal pHLuorin and cytosolic mkate2

-

-

BL-0987

f(syn)-NLS.RFP-P2A-SEP-GluR1

hSynapsin1

GluR1, pHluorin

Expression of nuclear RFP linked via P2A to GluR1 fused to super-ecliptic pHlourin, subclone of addgene# 24000

Addgene #24000

Kopec et al. 2006

BL-0988

f(syn)-NLS.RFP-P2A-SEP-GluR2

hSynapsin1

GluR2, pHluorin

Expression of nuclear RFP linked via P2A to GluR2 fused to super-ecliptic pHlourin, subclone of addgene# 24001

Addgene #24001

Kopec et al. 2006

BL-1849

f(syn)syphy-tdimer2

hSynapsin1

Synaptophysin, pHluorin

Expression of Synaptophysin-pHluorin and tdimer2 for ratiometric imaging. Subclone of addgene #44268.

Addgene #44268

Rose et al. 2013

Neurotransmitter and ATP sensors

Catalog #

Vector name

Promoter

Gene of Interest

Discription

MTA informations

Ref for publication

BL-1363

f(syn)GRAB-DA1h

hSynapsin1

GRAB-DA1h

Expression of the fluorescent dopamine(DA) sensor GRAB_DA1h in neurons. Subclone of addgene #113050

Addgene #113050

Sun et al. 2018

BL-1364

f(syn)GRAB-DA1m

hSynapsin1

GRAB-DA1h

Expression of the fluorescent dopamine(DA) sensor GRAB_DA1m in neurons. Subclone of addgene #113049

Addgene #113049

Sun et al. 2018

BL-1366

f(syn)dLight1.1

hSynapsin1

dLight1.1

Expression of the dopamine(DA) indicator dLight1 in neurons. Subclone of addgene #111053

Addgene #111053

Patriarchi et al. 2018

BL-1843

f(syn)Switch(SynATP)-w

hSynapsin1

SynATP

Cre-dependent expression (CreON) of an optical reporter of presynaptic ATP. Subclone of addgene #111053

Addgene #51819

Rangaraju et al. 2014

BL-1844

f(syn)Flox(SynATP)-w

hSynapsin1

SynATP

Cre-dependent expression (CreOFF) of an optical reporter of presynaptic ATP. Subclone of addgene #111053

Addgene #51819

Rangaraju et al. 2014

Optogenetic actuators

Catalog #

Vector name

Promoter

Gene of Interest

Discription

MTA informations

Ref for publication

BL-0336

f(syn)-Phönix-w

hSynapsin1

Halorhodopsin, SypHy and mkate2

Halorhodopsin fused to SypHy and mkate2 (synaptic vesicle proton pump and pH sensor)

Addgene #70111

Rost et al. 2016

BL-0347

f(syn)-ChR2-E123T/T159C-YFP-w

hSynapsin1

ChR2 (ChetaTC)

ChR2 (ChetaTC) fused to YFP. Subclone of addgene #35511

Addgene #35511

Bernd et al. 2011 and Mattis et al. 2011

BL-0365

f(syn)-CreON(ChR2-E123T/T159C-YFP)-w

hSynapsin1

ChR2 (ChetaTC)

CreON Vector: ChR2 (ChetaTC) fused to YFP. Subclone of addgene #35511

Addgene #35511

Bernd et al. 2011 and Mattis et al. 2011

BL-0370

f(syn)-SWITCH(RFP_revChR2-E123T/T159C-YFP)-w

hSynapsin1

ChR2 (ChetaTC

CreON/OFF Vector: Expression of RFP in Non-Cre cells, Expression of ChR2 (ChetaTC) in Cre cells. Subclone of addgene #35511

Addgene #35511

Bernd et al. 2011 and Mattis et al. 2011

BL-0644

f(syn)-CreON(NLS.RFP-P2A-ChR2-ET/TC)-w

hSynapsin1

ChR2 (ChetaTC

CreOn Vector: NLS.RFP linked to fast ChR2 (ChetaTC) via P2A, Cre dependent expression, subclone of addgene #35511

Addgene #35511

Bernd et al. 2011 and Mattis et al. 2011

BL-0662

f(syn)-CreOFF(ChR2-E123T/T159C-YFP)-w

hSynapsin1

ChR2 (ChetaTC

CreOFF Vector: Expression of ChR2 (ChetaTC) in Non-Cre cells. Subclone of addgene #35511

Addgene #35511

Bernd et al. 2011 and Mattis et al. 2011

BL-0679

f(syn)-ChR2-H134R-YFP-w

hSynapsin1

Chr2-H134R

Expression of Chr2-H134R fused to YFP, subclone of addgene #26973

Addgene #26973

-

BL-0693

f(syn)-ChR2-E123T/T159C-YFP-P2A-MCS-w

hSynapsin1

ChR2 (ChetaTC)

ChR2 (ChetaTC) fused to YFP and linked via a P2A sequence to a multiple cloning site (MSC). Subclone of addgene #35511

Addgene #35511

Bernd et al. 2011 and Mattis et al. 2011

BL-0792

f(syn)-VChR1-eYFP-w

hSynapsin1

ChR1

Expression of Red-shifted volvox ChR1 fused to YFP, subclone of addgene #20955

Addgene #20955

Zhang et al. 2008

BL-0903

f(CamKII)-ChrimsonR-GFP-w

CamKII

ChrimsonR

Expression of ChrimsonR fused to GFP

Addgene #59049

Klapoetke et al. 2014

BL-1847

f(syn)ChR2-ET/TC-mscarlet

hSynapsin1

ChR2 (ChetaTC)

ChR2 (ChetaTC) fused to mScarlet. Subclone of addgene #35511

Addgene #35511

Bernd et al. 2011 and Mattis et al. 2011

BL-1848

f(syn)ChR2-ET/TC-Cerulean3

hSynapsin1

ChR2 (ChetaTC)

ChR2 (ChetaTC) fused to Cerulean3. Subclone of addgene #35511

Addgene #35511

Bernd et al. 2011 and Mattis et al. 2011

Optical switches

Catalog #

Vector name

Promoter

Gene of Interest

Discription

MTA informations

Ref for publication

BL-0735

f(syn)-mCherry-Cry2PHR-w

hSynapsin1

Cryptochrome2

Expression of mCherry tagged Cryptochrome2 (aa1-498), subclone from addgene #28244

Addgene #28244

Kennedy et al. 2010

BL-0726

f(syn)-CIBN-GFP-TM-Stx1a-w

hSynapsin1

CIB1

Expression of a truncated version of a basic helix-loop-helix protein Arabidopsis CIB1 (1-170) fused to GFP and the transmembrane domain of Syntaxin1a, subclone of addgene #28246

Addgene #28246

Kennedy et al. 2010

BL-0732

f(syn)-CIBN-TM-Stx1a-w

hSynapsin1

CIB1

Expression of a truncated version of a basic helix-loop-helix protein Arabidopsis CIB1 fused to the transmembrane domain of Syntaxin1a, subclone of addgene #28246

Addgene #28246

Kennedy et al. 2010

BL-0802

f(syn)-mCherry-Cry2PHR-P2A-MCS-w

hSynapsin1

Cryptochrome2

Expression of mCherry tagged Cryptochrome2 (aa1-498) linked to a MCS via P2A, subclone from addgene #28244

Addgene #28244

Kennedy et al. 2010

BL-1142

f(syn)nMagHigh-eGFP-CAAX

hSynapsin1

VIVID

Blue light dependent photoswitching system: membrane bound nMag-eGFP. Subclone of addgene #67300

Addgene #67300

Kawano et al. 2015

BL-1136

f(syn)pMagFast2(3x)-iRPF

hSynapsin1

VIVID

Blue light dependent photoswitching system: soluble pMag-iRFP. Subclone of addgene #67297

Addgene #67297

Kawano et al. 2015

BL-1312

f(syn)CRY2PHR-mCherryN1

hSynapsin1

Cryptochrome2

Expression of the photolyase homology region (PHR) of cryptochrome 2 (Cry2, aa1-498) fused c-terminally to mCherry under control of the human synapsin1 promoter. Subclone of addgene #26866.

Addgene #26866

Kennedy et al. 2010

BL-1315

f(syn)CIBN(deltaNLS)-pmGFP

hSynapsin1

CIB1

Expression of the helix-loop-helix protein Arabidopsis CIB1 protein (aa1-170 with mutation of predicted NLS.Sequence fused to plasma targeted GFP (CAAX). Subclone of addgene #26867

Addgene #26867

Kennedy et al. 2010

BL-1313

f(syn)CRY2olig-mCherry

f(syn)

Cryptochrome2-Olig

Expression of the photolyase homology region (PHR) of cryptochrome 2 (Cry2, aa1-498 with E490G mutation (olig)) fused c-terminally to mCherry under control of the human synapsin1 promoter. Subclone of addgene #60032.

Addgene #60032

Taslimi et al. 2016

BL-1314

f(syn)CRY2PHR(W349R)-mCherry

f(syn)

CRY2PHR(W349R)

Expression of the photolyase homology region (PHR) of cryptochrome 2 (Cry2, aa1-498 with W349R mutation (fast-cycling)) fused c-terminally to mCherry under control of the human synapsin1 promoter. Subclone of addgene #75370.

Addgene #75370

Taslimi et al. 2016

General FP expression vectors

Catalog #

Vector name

Promoter

Gene of Interest

Discription

MTA informations

Ref for publication

BL-0100

f(syn)-GFP-P2A-MSCw

hSynapsin1

GFP

cytosolic GFP expression restricted to neurons (fused to a P2A sequence)

-

-

BL-0110

f(syn)-mkate2-P2A-MSCw

hSynapsin1

mkate2

mkate2 expression restricted to neurons (fused to a P2A sequence)

-

-

BL-0140

f(syn)-NLS.GFP-P2A-MSCw

hSynapsin1

NLS.GFP

nulcear localized GFP expression restricted to neurons (fused to a P2A sequence)

-

-

BL-0181

f(syn)-NLS.RFP-P2A-MSCw

hSynapsin1

NLS.RFP

nulcear localized RFP expression restricted to neurons (fused to a P2A sequence)

-

-

BL-0512

f(syn)-tdTomato-P2A-MSC

hSynapsin1

tdtomato

tdtomato expression restricted to neurons (fused to a P2A sequence)

-

-

BL-0526

f(CMV)-RFPw

CMV

RFP

CMV promoter controlled cytosolic RFP expression

-

-

BL-0533

f(CMV)-GFPw

CMV

GFP

CMV promoter controlled cytosolic GFP expression

-

-

BL-0534

f(CMV)-NLS.GFP-P2A-MSCw

CMV

NLS.GFP

CMV promoter controlled nuclear localized GFP expression (fused to a P2A sequence)

-

-

BL-0535

f(CMV)-NLS.RFP-P2A-MSCw

CMV

NLS.RFP

CMV promoter controlled nuclear localized RFP expression (fused to a P2A sequence)

-

-

BL-0540

f(GFAP2.2)-GFP-w

GFAP2.2

GFP

cytosolic GFP expression restricted in astrocytes

-

-

BL-0541

f(GFAP2.2)-NLS.GFP-P2A-MSCw

GFAP2.2

NLS.GFP

nuclear localized GFP expression restricted to astrocytes (fused to a P2A sequence)

-

-

BL-0542

f(GFAP2.2)-NLS.RFP-P2A-MSCw

GFAP2.2

NLS.RFP

nuclear localized RFP expression restricted to astrocytes (fused to a P2A sequence)

-

-

BL-0543

f(ubi)-NLS.GFP-P2A-MSCw

Ubiquitin

NLS.GFP

nuclear localized GFP expression (fused to a P2A sequence)

-

-

BL-0544

f(ubi)-NLS.RFP-P2A-MSCw

Ubiquitin

NLS.RFP

nuclear localized RFP expression (fused to a P2A sequence)

-

-

BL-0752

f(syn)-CFP-P2A-MSC

hSynapsin1

CFP

Cerulean expression (fused to a P2A sequence)

-

-

BL-1109

f(CAG)-GFP-w

CAG

GFP

CAG Promoter for cytosolic GFP expression

-

-

BL-1161

f(CAG)-NLS.GFP-w

CAG

NLS.GFP

CAG Promoter for nucklear localized GFP expression

-

-

BL-1181

f(syn)-NLS.CFP-P2A-w

hSynapsin1

NLS.CFP

nulcear localized CFP expression restricted to neurons (fused to a P2A sequence)

-

-

HL-0001

f(Ubi)-GFP-w

Ubiquitin

GFP

GFP expression under control of the Ubiquitin promoter (FUGW)

   

BL-1260

f(THP2.5)GFP-P2A-w

Tyrosine hydroxylse promoter (THP2.5)

GFP

GFP expression under control of the rat tyrosin hydroxylase promoter (2.5kb upstream, THP). Subclone of addgene clone #80336.

Addgene #80336

Oh et al. 2009

BL-1264

f(syn)-mKate-P2A-NLS.GFP

hSynapsin1

mkate2, NLS.GFP

Expression of mkate2 and NLS.GFP under the control of the human Synaptophysin1 promoter

-

-

BL-1388

CAG-NLS.RFP-P2A

CAG

NLS.RFP

CAG Promoter controlled nuclear localized RFP expression (fused to a P2A sequence)

-

-

BL-1643

f(CAG)-RFP-w

CAG

RFP

CAG Promoter controlled RFP expression

-

-

BL-1792

f(CMV)-RFP-P2A-MCS WPRE

CMV

RFP

CMV Promoter controlled RFP expression fused to a P2A and multiple cloning site.

-

-

Intracellular markers

Catalog #

Vector name

Promoter

Gene of Interest

Discription

MTA informations

Ref for publication

BL-0443

f(syn)mkate2-P2A-GFP-ER

hSynapsin1

mkate2, GFPer

Far red mkate2 linked via P2A to endosomal reticulum bound GFP

-

-

BL-0452

f(syn)eGFP-ER

hSynapsin1

GFPer

Endosomal reticulum bound GFP

-

-

BL-0500

f(syn)Gap-GFP-P2A

hSynapsin1

GAP-GFP

Membran bound GFP due to N-terminal fused dual palmitoylation sequence of GAP43

-

-

BL-0515

f(syn)tdtomato-P2A-GFP-ER

hSynapsin1

tdtomato, GFPer

tdtomato linked via P2A to endosomal reticulum bound GFP

-

-

BL-0569

f(syn)Tom20-GFP

hSynapsin1

Tom20

Mitochondrial Tom20 fused to GFP

-

-

BL-0625

f(syn)Rab5a-GFP-w

hSynapsin1

Rab5a

Mouse Rab5a fused to GFP

-

-

BL-0721

f(syn)w-PA-GFP-Actin

hSynapsin1

ß-Actin

Photoactivatable GFP variant fused to Actin, subclone from addgene plasmid 57120

Addgene #57210

Olenych et al. 2007

BL-0722

f(syn)Lifeact-mEOS3.2

hSynapsin1

lifeact

Lifeact-mEOS3.2 expression, subclone from addgene plasmid 54696 into LV vector.

Addgene #54696

-

BL-0758

f(syn)w-eGFP-2FYFE

hSynapsin1

FYFE

two FYFE domains fused to GFP (PI3P binder)

Addgene #36096

Burd et al. 1998

BL-0855

f(syn)LifeAct-GFP-w

hSynapsin1

lifeact

Lifeact-GFP expression, subclone from addgene plasmid 54696 into LV vector.

Addgene #54696

 

BL-1250

f(syn)LifeAct-mkate2-w

hSynapsin1

lifeact

Lifeact sequence fused to mkate2, subclone from addgene plasmid 54696 into LV vector.

Addgene #54696

 

BL-1282

f(syn)eGFP-Rab8-w

hSynapsin1

Rab8

Rab8 fused to GFP, subclone of addgene #31803.

Addgene #31803

Guizetti et al. 2011

BL-1467

pLEX-CEPIA1er

hPGK

CEPIA1er

Expression of ER-tagged CEPIA1 under the control of the hPGK Promoter. Subclone of Addgene #58216 in pLex_305 (Addgene #41390)

Addgene #58216 and Addgene #41390

Suzuki et al. 2014

BL-1469

pLEX-CEPIA3mt

hPGK

CEPIA3mt

Expression of MT-tagged CEPIA3 under the control of the hPGK Promoter. Subclone of Addgene #58219 in pLex_305 (Addgene #41390)

Addgene #58219 and Addgene #41390

Suzuki et al. 2014

BL-1468

pLEX-CEPIA2mt

hPGK

CEPIA2mt

Expression of MT-tagged CEPIA2 under the control of the hPGK Promoter. Subclone of Addgene #58218 in pLex_305 (Addgene #41390)

Addgene #58218 and Addgene #41390

Suzuki et al. 2014

BL-1470

pLEX-CEPIA4mt

hPGK

CEPIA4mt

Expression of MT-tagged CEPIA4 under the control of the hPGK Promoter. Subclone of Addgene #58220 in pLex_305 (Addgene #41390)

Addgene #58220 and Addgene #41390

Suzuki et al. 2014

BL-1738

f(syn)Flag-Cortactin

hSynapsin1

Cortactin

Expression of the actin binding protein Cortactin fused to Flag Tag. Subclone of Addgene #74476.

Addgene #74476

Weed et al. 2000

BL-1746

f(syn)emerald-Cortactin

hSynapsin1

Cortactin

Expression of the actin binding protein Cortactin fused to emerald. Subclone of Addgene #54051.

Addgene #54051

-

Synaptic markers

Catalog #

Vector name

Promoter

Gene of Interest

Discription

MTA informations

Ref for publication

BL-0141

f(syn)-NLS.GFP-P2A-VGlut1mkate2

hSynapsin1

Vglut1

Nuclear GFP linked via P2A to VGlut1 fused to mkate2

-

-

BL-0177

f(syn)-Syp-GFP-w

hSynapsin1

Synaptophysin

Synaptophysin fused to GFP

-

Sampathkumar et al. 2016

BL-0195

f(syn)-Syp-GFP-P2A-MCS-w

hSynapsin1

Synaptophysin

Synaptophysin fused to GFP and a P2A Sequence

-

Sampathkumar et al. 2016

BL-0258

f(syn)-NLS.GFP-P2A-Syp-mkate2

hSynapsin1

Synaptophysin

Nuclear GFP linked via P2A to Synaptophysin fused to mkate2

-

Sampathkumar et al. 2016

BL-0373

f(syn)-Syp-mkate2-w

hSynapsin1

Synaptophysin

Synaptophysin fused to mkate2

-

Sampathkumar et al. 2016

BL-0418

f(syn)-VGlut1-mkate2-w

hSynapsin1

VGlut1

VGlut1 fused to mkate2

-

-

BL-0492

f(syn)-VGlut1-GFP-w

hSynapsin1

VGlut1

VGlut1 fused to GFP

-

-

BL-0760

f(syn)-NLS.RFP-P2A-Syp-CFP-T2A-MCS

hSynapsin1

Synaptophysin

Nuclear RFP linked via P2A to Synaptophysin fused to CFP and linked to a second 2A site with a Multiple Cloning Site

-

-

BL-0856

f(syn)-LifeAct-GFP-P2A-Syp-mkate2

hSynapsin1

lifeact, Synaptophysin

Expression of Lifeact-GFP linked via P2A to Synaptophysin fused to mkate2, subclone from addgene #54696

Modifiction of Addgene #54696

-

BL-1033

f(syn)-Homer1-GFP-w

hSynapsin1

Homer1

Expression of postsynaptic Homer fused to GFP, subclone of addgene #57461

Addgene #57461

-

BL-1034

f(syn)-Homer1-tdtomato

hSynapsin1

Homer1

Expression of postsynaptic Homer fused to tdtomato, subclone of addgene #57461

Addgene #57461

-

BL-1035

f(syn)-Homer1-mEOS3.2-w

hSynapsin1

Homer1

Expression of postsynaptic Homer fused to mEOS3.2, , subclone of addgene #57461

Addgene #57461

-

BL-1038

f(syn)-GluR1-mEOS3.2

hSynapsin1

GluR1

Expression of postsynaptic GluR1 labeled with extracellulär mEOS3.2, subclone of addgene #57461

Addgene #57461

-

BL-1054

f(syn)-GluR2-mEOS3.2

hSynapsin1

GluR2

Expression of postsynaptic GluR2 labeled with extracellulär mEOS3.2, subclone of addgene #57461

Addgene #57461

-

BL-1122

f(syn)-Homer1-CFP-w

hSynapsin1

Homer1

Expression of postsynaptic Homer fused to CFP, , subclone of addgene #57461

Addgene #57461

-

BL-1823

f(syn)GFP-P2A_Sypmkate2

hSynapsin1

Synaptophysin

Expression of Synapotphysin fused to mkate2 and cytosolic GFP.

-

-

Marker of synaptic connections

Catalog #

Vector name

Promoter

Gene of Interest

Discription

MTA informations

Ref for publication

BL-0592

f(syn)-post-mGRASP-2A-dTomato-w

hSynapsin1

post-mGRASP

For mapping synaptic connectivity: postsynaptic mGRASP linked via 2A to dTomato (subclone from addgene clone #34912)

Addgene #34912

Kim et al. 2011

BL-0593

f(syn)-pre-mGRASP-mCerulean-2A-NLS.mCherry-w

hSynapsin1

pre-mGRASP

For mapping synaptic connectivity: presynaptic mGRASP fused to mCerulean and linked via 2A to NLS.mcherry (subclone from addgene clone #34911)

Addgene #34911

Kim et al. 2011

BL-0638

f(syn)-NLS.RFP-P2A-post-mGRASP-w

hSynapsin1

post-mGRASP

For mapping synaptic connectivity: Nuclear RFP linked via P2A with postsynaptic mGRASP (subclone from addgene clone #34912)

Addgene #34912

Kim et al. 2011

BL-0639

f(syn)-NLS.GFP-P2A-pre-mGRASP-mkate2-w

hSynapsin1

pre-mGRASP

For mapping synaptic connectivity: Nuclear GFP linked via P2A with presynaptic mGRASP fused to mkate2 (subclone from addgene clone #34911)

Addgene #34911

Kim et al. 2011

Lipid marker

Catalog #

Vector name

Promoter

Gene of Interest

Discription

MTA informations

Ref for publication

BL-0725

f(syn)w iRFP-PH (PI4,5P2)

hSynapsin1

PHD - PLCdelta1

PI4,5P2 marker: PH domain of human PLCdelta1 fused to iRFP

Addgene #36075 und Addgene #54786

Chisari et al., 2009 and Filinov et al., 2011

BL-1446

f(syn)PLCd(PHD)-eGFP-P2A.WPRE (PI4,5P2)

hSynapsin1

PHD - PLCdelta1

PI4,5P2 marker: PH domain of human PLCdelta1 fused to eGFP-P2A (subclone from addgene clone #51407)

Addgene #51407

P. Varnai and T. Balla, 1998

BL-1493

f(syn)PLCd(PHD)-mCherry-P2A.WPRE (PI4,5P2)

hSynapsin1

PHD - PLCdelta1

PI4,5P2 marker: PH domain of human PLCdelta1 (against PIP4,5) fused to mCherry-P2A (subclone from addgene clone #51407)

Addgene #51407

P. Varnai and T. Balla, 1998

BL-1453

f(Syn)GFP-GRP1(PHD)-w

hSynapsin1

PHD -GRP1

PIP3 marker: PH domain of GRP1 fused to GFP. Subclone of addgene clone #71378

Addgene #71378

Kavran et al., 1998

BL-1447

f(syn)Akt.PHD-RFP-w (PIP3)

hSynapsin1

PHD - AKT

PIP3 marker: PH domain of AKT fused to RFP. Subclone of addgene clone #51465

 

Addgene #51465/a>

P. Varnai and T. Balla, 1998

BL-1450

f(syn)mcherry-DrrA-w

hSynapsin1

DrrA / SidM

Biosensor for PtdIns4P fused to mCherry. Subclone of addgene clone #51471

Addgene #51471/a>

Hammond et al., 2014

BL-1431

f(syn)D4H-mCherry-w (Cholesterol)

hSynapsin1

D4H

A cholesterol biosensor, termed D4H, based on the fourth domain of Clostridium perfringens theta-toxin. Fusion with mCherry. Subclone into f(syn)w.

D4H-mCherry-w was a gift from Greg Fairn.

Maekawa & Fairn, 2015

BL-1492

f(syn)D4H-GFP-w (Cholesterol)

hSynapsin1

D4H

A cholesterol biosensor, termed D4H, based on the fourth domain of Clostridium perfringens theta-toxin. Fusion with GFP. Subclone of BL-1431.

D4H-mCherry-w was a gift from Greg Fairn.

Maekawa & Fairn, 2015

Cre/FLP recombinase vectors

Catalog #

Vector name

Promoter

Gene of Interest

Discription

MTA informations

Ref for publication

BL-0150

f(syn)iCreRFP-P2A-MSCw

hSynapsin1

iCre-RFP

Synapsin promoter dependent expression of Cre recombinase fused to a RFP-P2A sequence

-

Vardar et al. 2016

BL-0224

f(syn)iCreCFP-P2A-MSCw

hSynapsin1

iCre-CFP

Synapsin promoter dependent expression of Cre recombinase fused to a CFP-P2A sequence

-

-

BL-0300

f(syn)iCre-P2A-MSCw

hSynapsin1

iCre

Synapsin promoter dependent expression of Cre recombinase fused to a P2A sequence

-

-

BL-0366

f(syn)iCreGFP-P2A-MSCw

hSynapsin1

iCre-GFP

Synapsin promoter dependent expression of Cre recombinase fused to a GFP-P2A sequence

-

-

BL-0445

f(syn)FLP-GFP-P2A-MSCw

hSynapsin1

FLP-GFP

Synapsin promoter dependent expression of FLP recombinase fused to GFP-P2A sequence

-

-

BL-0554

f(CMV)iCreRFP-P2A-MSCw

CMV

iCre-RFP

CMV promoter dependent expression of Cre recombinase fused to a RFP-P2A sequence

-

-

BL-0555

f(CMV)iCreGFP-P2A-MSCw

CMV

iCre-GFP

CMV promoter dependent expression of Cre recombinase fused to a GFP-P2A sequence

-

-

BL-0556

f(CMV)iCreCFP-P2A-MSCw

CMV

iCre-CFP

CMV promoter dependent expression of Cre recombinase fused to a CFP-P2A sequence

-

-

BL-0558

f(GFAP2.2)iCreRFP-P2A-MSCw

GFAP2.2

iCre-RFP

GFAP (2.2) promoter dependent expression of Cre recombinase fused to a RFP-P2A sequence

-

-

BL-0596

f(ubi)iCreRFP-P2A-MSCw

Ubiquitin

iCre-RFP

hUbiquitin promoter dependent expression of Cre recombinase fused to a RFP-P2A sequence

-

-

BL-0597

f(ubi)iCreGFP-P2A-MSCw

Ubiquitin

iCre-GFP

hUbiquitin promoter dependent expression of Cre recombinase fused to a GFP-P2A sequence

-

-

BL-0598

f(ubi)iCreCFP-P2A-MSCw

Ubiquitin

iCre-CFP

hUbiquitin promoter dependent expression of Cre recombinase fused to a CFP-P2A sequence

-

-

BL-1644

f(CAG)iCre-RFP-P2A.w

CAG

iCre-RFP

CAG promoter dependent expression of Cre recombinase fused to a RFP-P2A sequence

-

-

Cre vectors with marker genes

Catalog #

Vector name

Promoter

Gene of Interest

Discription

MTA informations

Ref for publication

BL-0155

f(syn)iCreRFP-P2A-SypGFP

hSynapsin1

iCre-RFP, Synaptophysin-GFP

iCre-RFP and Synaptophysin-GFP expression coupled via P2A

-

-

BL-0290

f(syn)iCreRFP-P2A-SypmKate2

hSynapsin1

iCre-RFP, Synaptophysin-mkate2

iCre-RFP and Synaptophysin-mkate2 expression coupled via P2A

-

-

BL-0291

f(syn)iCreCFP-P2A-Sypmkate2

hSynapsin1

iCre-CFP, Synaptophysin-mkate2

iCre-CFP and Synaptophysin-mkate2 expression coupled via P2A

-

-

BL-0408

f(syn)iCre-P2A-SypGFP

hSynapsin1

iCre, Synaptophysin-GFP

iCre and Synaptophysin-GFP expression coupled via P2A

-

-

BL-1570

f(syn)iCre.P2A-EGFP WPRE

hSynapsin1

iCre, eGFP

iCre and eGFP expression under control of the human Synapsin1 promoter.

-

-

BL-1571

f(CAG)iCre.P2A-EGFP WPRE

CAG

iCre, eGFP

iCre and eGFP expression under control of the hybrid CAG promoter.

-

-

             

BL-1532

f(syn)Cre-P2A-PSD95-mCherry

hSynapsin1

iCre, PSD95-mCherry

iCre and PSD95-mCherry expression coupled via P2A

-

-

BL-1508

f(syn)Cre-P2A-Syp-mCherry

hSynapsin1

iCre, Syp-mCherry

iCre and Synaptophysin-mCherry expression coupled via P2A

-

-

Cre dependent cloning vectors

Catalog #

Vector name

Promoter

Gene of Interest

Discription

MTA informations

Ref for publication

BL-0250

f(syn)SWITCH(NLS.GFP-P2A-MCS_revRFP)

hSynapsin1

NLS.GFP, RFP

Cre-ON/OFF Vector: Expression of nuclear localized GFP (fused to P2A) in non-Cre expressing cells, Expression of RFP in Cre rexpressing cells.

-

Chang et al. 2014

BL-0251

f(syn)SWITCH(RFP_revNLS.GFP-P2A-MCS)

hSynapsin1

NLS.GFP, RFP

Cre-ON/OFF Vector: Expression of RFP in non-Cre expressing cells, Expression of nuclear GFP in Cre rexpressing cells.

-

Chang et al. 2014

BL-0261

f(syn)CreOFF(NLS.GFP-P2A-MCS)

hSynapsin1

NLS.GFP

Expression of nuclear localized GFP in non-Cre expressing cells (GFP is fused to a P2A sequence)

-

Chang et al. 2014

BL-0315

f(syn)CreOFF(NLS.RFP-P2A-MCS)

hSynapsin1

NLS.RFP

Expression of nuclear localized RFP in non-Cre expressing cells (GFP is fused to a P2A sequence)

-

Chang et al. 2014

BL-0320

f(syn)CreON(NLS.GFP-P2A-MCS)

hSynapsin1

NLS.GFP

Expression of nuclear localized GFP (fused to P2A) in Cre expressing cells

-

Chang et al. 2014

BL-0350

f(syn)CreON(NLS.RFP-P2A-MCS)

hSynapsin1

NLS.RFP

Expression of nuclear localized RFP (fused to P2A) in Cre expressing cells

-

Chang et al. 2014

IPSC reprogramming vectors

Catalog #

Vector name

Promoter

Gene of Interest

Discription

MTA informations

Ref for publication

BL-851

TetO-FUW-AsclI-Puro

TetO

AsclI

Expresses AsclI and puromycin resistent gene under control of TetON promoter.

Addgene #97329

Yang et al. 2017

BL-1308

TetO-FUW-Sox2-IRES-Hygro

TetO

Sox2

Expresses Sox2 and Hygromycin resistent gene under control of TetON promoter. Sublcone of Addgene #20326

Addgene #20326

Brambrink et al. 2008

BL-1287

TetO-FUW-Dlx2-IRES-Hygro

TetO

Dlx2

Expresses Dlx2 and Hygromycin resistent gene under control of TetON promoter.

Addgene #97330

Yang et al. 2017

BL-1525

TetO-FUW-Hygro

TetO

Hygromycin

Expresses Hygromycin resistent gene under control of TetON promoter.

-

-

BL-1587

TetO-FUW-Dlx2-IRES-Blast

TetO

Dlx2

Expresses Dlx2 and Blasticidin S deaminase of Aspergillus terreus (BSD) under control of TetON promoter. Subclone from BL-1287.

Addgene #97330

Yang et al. 2017

BL-1600

TetO-FUW-DLX2.P2A-AsclI-IRES.Puro

TetO

Dlx2, AsclI

Expresses Dlx2, AsclI and puromycin resistent gene under control of TetON promoter. Subclone from Addgene #97329 and #97330

Addgene #97329 and Addgene #97330

Yang et al. 2017

BL-1812

FU-tetO-ASCL1-LMX1b-NURR1-PuroR

TetO

ASCL1, LMX1B, NURR1

Expresses AsclI, LMX1b, NURR1 (ALN TFs) and puromycin resistent gene under control of TetON promoter. Addgene #182298

Addgene #182298

Powell et al. 2021

CRISPR vectors

Catalog #

Vector name

Promoter

Gene of Interest

Discription

MTA informations

Ref for publication

BL-1300

U6-sgRNA-empty-TRE-HifiCas9(VP12)-P2A-hygro

U6 promoter, TetO

Cas9

High-fidelty Cas9 (VP12) expression under control of TetOn Promoter, empty U6 sgRNA expression cassette and hygromycin resistent gene, Subclone of Addgene #72247.

Addgene #72247

Kleinstiver et al. 2016

BL-1269

U6-sgRNA-empty-TRE-HifiCas9(VP12)

U6 promoter, TetO

Cas9

High-fidelty Cas9 (VP12) expression under control of TetOn Promoter and with empty U6 sgRNA expression cassette, Subclone of Addgene #72247.

Addgene #72247

Kleinstiver et al. 2016

Tracing helper vectors

Catalog #

Vector name

Promoter

Gene of Interest

Discription

MTA informations

Ref for publication

BL-1255

pLenti-CMV-GFP-2A-oG-WPRE

CMV

Rabies Gylcoprotein oG, GFP

addgene 102984

Addgene #102984

Ciabatti al. 2017

BL-1256

pLenti-CMV-puro-2A-TEVp

CMV

TEV-protease, Puromycin

addgene 99610

Addgene #99610

Ciabatti al. 2017

BL-1722

f(CAG)VSV.G-IRES-NLS-GFP-P2A-Puro-w

CAG

VSV Glycoprotein, Puromycin

Expression of VSV-G. NLS-GFP and Puromycin

-

-

BL-1724

f(CAG)VSV.G-IRES-NLS-GFP-w

CAG

VSV Glycoprotein,

Expression of VSV-G and NLS-GFP

-

-

BL-1846

f(CAG)VSV.G-IRES-CFP3-w

CAG

VSV Glycoprotein,

Expression of VSV-G and CFP3

-

-

last update: 08.02.2023