Catalog of RABV and SFV Vectors

Here you will find an overview of our different RABV and SFV vectors and existing viruses (ready-made). You can enter the corresponding catalog number in our order system and have your desired virus produced.

External users must complete an MTA between the Charité and its facility, and for some vectors, appropriate Addgene regulations must be applied. You will receive further information during the ordering process.

 

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Rabies Virus (RABV)

At this point you will find the various Rabies viruses offered. Ready-Made Rabies viruses are all EnvA pseudotyped and concentrated by ultracentrifugation.

Catalog #

Vector name

Promoter

Discription

ready-made RABV

MTA informations

Ref for publication

BRABV-001

pSADB19dG-mCherry

RABV-B19

cytosolic mCherry expression

yes

-

-

BRABV-002

pSADB19dG-GFP

RABV-B19

cytosolic GFP expression

yes

   

BRABV-007

pSADB19dG-iCre-RFP

RABV-B19

Codon improved Cre recombinase fused to RFP

-

-

-

BRABV-008

pSADB19dG-ChR2-ET/TC-YFP

RABV-B19

Expression of ChR2 (ChetaTC) fused to YFP, Subclone of addgene #35511

-

Addgene #35511

Bernd et al. 2011 and Mattis et al. 2011

BRABV-009

pSADB19dG-GCaMP6

RABV-B19

Expression of GCaMP6f

-

Addgene #40755

Chen et al. 2013

AAV Vectors essential for the application of RABV

Catalog #

Vector name

Promoter

Discription

Serotypes of Ready-mades

additional MTA requirements

Ref for publication

BA-064

pAAV-EF1a-DIO-HTB

EF1alpha

Cre dependent expression of Histone tagged GFP linked via T2A to TVA receptor linked via E2A to Rabies B19G

-

MTA for Addgene #44187

-

BA-068

pAAV-Ef1a-DIO-H2B-GFP-2A-oG-WPRE-hGG

EF1alpha

Cre dependent expression of Histone tagged GFP linked via T2A to optimized Rabies B19G (oG)

-

MTA for Addgene #74289

Kim et al. 2016

BA-091

pAAV-Syn-H2B-GFP-TVA-oG-WPRE3

hSynapsin1, WPRE3

Enhanced Expression of Histone tagged GFP linked via T2A to TVA receptor linked via E2A to Rabies B19oG

AAV1

MTA for Addgene #74289

Kim et al. 2016

BA-096

pAAV-Syn-FLEX-nGToG-WPRE3

hSynapsin1

Enhanced Cre dependent expression of nuclear localized (NLS) GFP linked via T2A to TVA receptor linked via E2A to Rabies B19oG

AAV1

MTA for Addgene #74289

Kim et al. 2016

Semliki Forest Virus

Catalog #

Vector name

Promoter

Discription

MTA informations

Ref for publication

 

BSFV-003

pSFV-1-GFP

SFV

cytosolic GFP expression

-

-

 

BSFV-004

pSFV-1-ChR2-H134R-eYFP

SFV

Expression of Chr2-H134R fused to YFP, subclone of addgene #26973

Addgene #26973

-

 

BSFV-005

pSFV-1-CaMPARI

SFV

Expression of CaMPARI, a calcium-modulated photoactivatable ratiometric integrator. Subclone of addgene #60421

Addgene #60421

Fosque et al. 2015 and Zolnik et al. 2017