Vector DNA Service
In the DNA service, we offer you the service of tailor-made generation of new viral plasmid vectors. Standard cloning techniques and the latest recombination techniques are used. Finished vectors are sequenced throughout and amplified for subsequent virus production in sufficient quantities in bacteria.
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Cost Vector DNA Service
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Service |
Cost |
Discription |
|---|---|---|
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Clone new DNA viral expression vectors (one step, cDNA is provided) |
755 € Intern, 800 € BUA*, 850 € Extern |
Includes: primer design, DNA amplification by PCR, ligation, transformation, minipreps, thorough restriction enzyme and DNA sequence analysis |
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Clone new DNA viral expression vectors (one step, no cDNA provided) |
1255 € Intern, 1300 € BUA*, 1500 € Extern |
Includes tissue specific generation of cDNA and amplification of gene of interest by PCR. (All other steps like above) |
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Clone new viral sh/sgRNA vectors (one step) |
650 € Intern, 700 € BUA*, 750 € Extern |
Includes generation of 2 sh/sgRNA vectors targeting the same gene. (All other steps like above) |
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Clone new adenoviral vector (cDNA is provided) |
965 € Intern, 1100 € BUA*, 1300 € Extern |
Includes: Primer design, DNA amplification by PCR, insertion into adenovireal backbone, transformation, minipreps and DNA sequence analysis |
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Clone new adenoviral vector |
1490 € Intern, 1600 € BUA*, 1800 € Extern |
Includes: tissue specific generation of cDNA and amplification of gene of interest by PCR. (All other steps like above) |
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Single steps |
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Plasmid DNA production |
105 € Intern, 140 € BUA*, 170 € Extern |
DNA for cell transfection. Includes DNA quantification and restriction enzyme analysis |
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E.coli transformation and Mini preparation |
50 € Intern, 60 € BUA*, 80 € Extern |
Plasmid DNA transformation and plasmid preparation of et least 4 clones |
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Plasmid sanger sequencing |
50 € Intern, 60 € BUA*, 80 € Extern |
sequencing of GOI (max 10kb) within a plasmid. |
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* BUA = Berlin University Alliance, research groups within the Berlin science network |
