Retrovirus

  • Infection of dividing cells
  • long-lasting transgene expression, genome-integrating
  • 8 kb DNA uptake capacity
  • 2 ssRNA genome
  • about 100 nm capsid size
  • Safety level S1 for ecotropic retroviral particles 

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Retroviral vectors and retrovirus production

As retroviral vectors we are currently using a MSCV (murine stem cell virus) based retroviral expression system, in which a 5'LTR from the murine stem cell PCMV virus drives high-level, constitutive expression of the transgene in stem cells and other mammalian cell lines.

For generating puromycin resistant cell lines you can choose a second type of vector, which contains a PGK promoter driven puro expression cassette.

Retroviral production is performed by transfection of the retroviral vector into the packaging cell line BOSC23; a HEK293T cell line into which retroviral packaging functions of Moloney Murine Leukemia Virus (MoMLV) were stably introduced. Using this cell line we can generate high titers of ecotropic retroviral particles, which stably integrate into proliferating mouse and rat cells (S1 Level).

Costs - Retrovirus production

Service

Costs

Discription

Retroviral production in small-scale

200€

Retrovirale Particle production in BOSC23 cells. Scale: 6ml, app. 10^6 particles / ml

Retroviral production in medium-scale

300€

Retrovirale Particle production in BOSC23 cells. Scale: 12ml, app. 10^6 particles / ml