- Infection of dividing cells
- long-lasting transgene expression, genome-integrating
- 8 kb DNA uptake capacity
- 2 ssRNA genome
- about 100 nm capsid size
- Safety level S1 for ecotropic retroviral particles
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Retroviral vectors and retrovirus production
As retroviral vectors we are currently using a MSCV (murine stem cell virus) based retroviral expression system, in which a 5'LTR from the murine stem cell PCMV virus drives high-level, constitutive expression of the transgene in stem cells and other mammalian cell lines.
Retroviral production is performed by transfection of the retroviral vector into the packaging cell line BOSC23; a HEK293T cell line into which retroviral packaging functions of Moloney Murine Leukemia Virus (MoMLV) were stably introduced. Using this cell line we can generate high titers of ecotropic retroviral particles, which stably integrate into proliferating mouse and rat cells (S1 Level).