Katalog der AAV Vektoren und Viren

Hier finden Sie eine Übersicht zu unseren verschiedenen AAV Plasmid-Vektoren und bereits vorhandenen Viren (Ready-made). Sie können in unserem Bestellsystem die entsprechende Katalognummer eintragen und ihren gewünschten Virus produzieren lassen.

Externe Nutzer müssen einen MTA zwischen der Charité und ihrer Einrichtung abschließen und bei bei einigen Vektoren müssen entsprechende Addgene Regularien angewendet werden. Nähere Informationen erhalten Sie hierzu während des Bestellprozesses.

 

 

 

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AAV Werkzeuge für Ihre Wissenschaft

Mit welchem Vektor/Virus können wir Ihnen helfen? Sortiert nach ihren spezifischen Transgenen, finden Sie hier verschiedenste Werkzeuge, die Ihnen in Ihrer wissenschaftlichen Fragestellung helfen könnte. Falls ein Vektor für Sie interessant ist, können Sie ihn als regular oder large Scale in unserem Ordersystem bestellen. Wenn der Vektor bereits als "ready-made" Virus in unseren Stocks existiert, können wir ihn für Pilot Studien schnell zur Verfügung stellen.

Wir versuchen die Liste immer aktuell zu halten. Kontaktieren sie uns bitte falls ihr gewünschter Vektor nicht dabei ist. Nutzen Sie unseren DNA Service falls wir vorhandene Vektoren für Sie spezifisch modifizieren könnten.

Optogenetische Sensoren für Neurowissenschaften

Catalog # Vector name Promoter Discription available as "ready-made" AAV MTA informations Ref for publication
BA-025 pAAV-hSyn-mCherry-P2A-GCaMP6f hSynapsin1 Expression of mCherry linked via a P2A sequence to GCaMP6f, sublcone of addgene #40755 no Addgene #40755 Chen et al. 2013
BA-026 pAAV Syn ruby-P2A-GCamp6f hSynapsin1 Expression of Ruby linked via a P2A sequence to GCaMP6f yes, Serotype AAV9 Addgene #50943 Rose et al. 2016
BA-027 pAAV Syn ruby-P2A-GCamp6s hSynapsin1 Expression of Ruby linked via a P2A sequence to GCaMP6s no Addgene #50942 Rose et al. 2016
BA-037 pAAV-hSyn-CaMPARI-w hSynapsin1 Expression of CaMPARI, a calcium-modulated photoactivatable ratiometric integrator. Subclone of addgene #60421 no Addgene #60421 Fosque et al. 2015 and Zolnik et al. 2017
BA-038 pAAV-hSyn-Flex-CaMPARI-w hSynapsin1 Cre-dependent expression of CaMPARI, a calcium-modulated photoactivatable ratiometric integrator. yes, Serotype AAV2retro Addgene #60421 Fosque et al. 2015 and Zolnik et al. 2017
BA-077 pAAV-Syn-flex-NES-jRCaMP1b-MBD-WPRE3 hSynapsin1 Cre dependent expression of jRCamP1b localized to dentrites (Myosin Binding Domain, MBD). Subclone of addgene #63136 and #21484) yes, Serotype AAV9 Addgene #63136 and Addgene #21484 Dana et al. 2016 and Lewis et al. 2009
BA-079 pAAV-Syn-flex-NES-jRCaMP1b-WPRE3 hSynapsin1 Enhanced Cre dependent expression of the Red fluorescent calcium sensor protein jRCaMP1b, subclone of addgene #63136 yes, Serotype AAV9 Addgene #63136 Dana et al. 2016
BA-095 pAAV Syn Flex GCamp6f-wpre3 hSynapsin1 Cre-dependent expression of GCaMP6f yes, Serotype AAV2retro Addgene #40755 Chen et al. 2013
BA-102 pAAV-RAM-d2TTA::TRE-MCS-WPRE-pA RAM/TRE Robust activity marking system for exploring active neuronal ensembles. Empty vector, use in combination with DNA service request. no Addgene #63931 Sorensen et al. 2016
BA-103 pAAV-RAM-d2TTA::TRE-FLEX-MCS-WPRE-pA RAM/TRE Robust activity marking system for exploring active neuronal ensembles. Empty vector - Cre dependent, use in combination with DNA service request. no Addgene #84467 Sorensen et al. 2016
BA-104 pAAV-RAM-d2TTA::TRE-EGFP-WPREpA RAM/TRE Robust activity marking system for exploring active neuronal ensembles. GFP Expressing no Addgene #84469 Sorensen et al. 2016
BA-107 AAV-Syn-ER-GCamp6-150-WPRE3 hSynapsin1 Fluorescent reporter for ER calcium signaling - Expression of ER-GCaMP6-150 no Addgene #86918 de Juan-Sanz et al. 2017
BA-108 AAV-Syn-ER-GCamp6-210-WPRE3 hSynapsin1 Fluorescent reporter for ER calcium signaling - Expression of ER-GCaMP6-210 no Addgene #86919 de Juan-Sanz et al. 2017
BA-110 pAAV-mDlx-GCaMP6f-Fishell-2 mDLX GCaMP6f expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element yes, Serotype AAV9, PHP.eB Addgene #83899/ Dimidschstein et al. 2016

Optogenetische Aktivatoren für Neurowissenschaften

Catalog # Vector name Promoter Discription available as "ready-made" AAV MTA informations Ref for publication
BA-003 pAAV-Syn-flex ChR2(H134R)-YFP-WPRE hSynapsin1 Cre recombinase dependent Expression of hChR2(H134R)-YFP, subclone of addgene #26973 yes, Serotype AAV1, AAV2retro Addgene #26973 -
BA-006 pAAV-hSyn-hCHR2(H134R)-eYFP-WPRE hSynapsin1 Expression of hChR2(H134R) fused to YFP for optical controling neuronal activation yes, Serotype AAV1, AAV2retro Addgene #26973 -
BA-007 pAAV-CAG-flex rev-Chr2wt-tdTomato -WPRE CAG Cre dependent expression of WT-hChR2 fused to tdTomato no Addgene #18917 Atasoy et al. 2008
BA-029 pACAGW-oChiEFtdTomato-AAV CAG Expression of oChIEF (chimera of ChR1 and ChR2) fused to tdTomato no Addgene #32846 Lin et al. 2009 and Jurgens et al. 2012
BA-030 pAAV-Flex-rev-oChIEF-tdTomato CAG Cre dependent expression of oChIEF (chimera of ChR1 and ChR2) fused to tdTomato yes, Serotype AAV2 Addgene #30541 Lin et al. 2009 and Bell et al. 2011
BA-031 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE EF1alpha Cre recombinase dependent Expression of hChR2(H134R) fused to YFP no Addgene #20298 -
BA-044 pAAV-CAG Flex-hChR2-T159C-P2A-eYFP CAG Cre dependent expression of Chr2 T159C linked via P2A to YFP, subclone of addgene #35511 no Addgene #35511 Bernd et al. 2011 and Mattis et al. 2011
BA-049 pAAV CAG Flex hChR2(H134R)-mCherry-WPRE CAG Cre dependent expression of hChR2(H134R) fused to mCherry for optical controling neuronal activation yes, Serotype AAV1, AAV6 Addgene #26973 -
BA-066 pAAV-CamKII-ReaChR-P2A-YFP CamKII Expression of a red-shifted variant of channelrhodopsin (ReaChR) linked via P2A to YFP no Addgene #50954 Lin et al. 2013
BA-074 pAAV-Syn-hChR2(H134R)-YFP-WPRE3 hSynapsin1, WPRE3 Enhanced expression of hChR2(H134R) fused to YFP for optical controling neuronal activation using a WPRE3 sequence, subclone of addgene #26973 and #61463 no Addgene #26973 and Addgene #61463 Choi et al. 2014
BA-078 pAAV-CMV-hChR2(H134R)-YFP.WPRE3 CMV, WPRE3 Enhanced expression of hChR2(H134R) fused to YFP for optical controling neuronal activation using a WPRE3 sequence, subclone of addgene #26973 and #61463 no Addgene #26973 and Addgene #61463 Choi et al. 2014
BA-080 pAAV-hSyn-ReaChR-p2A-mRuby hSynapsin1 Expression of a red-shifted variant of channelrhodopsin (ReaChR) linked via P2A to mRuby no Addgene #50954 Lin et al. 2013
BA-081 pAAV-hSyn-ReaChR-p2A-tdTomato hSynapsin1 Expression of a red-shifted variant of channelrhodopsin (ReaChR) linked via P2A to tdTomato no Addgene #50954 and für Addgene #40755 Lin et al. 2013
BA-084 pAAV-hSyn-ReaChR-p2A-gCamp6f hSynapsin1 Expression of a red-shifted variant of channelrhodopsin (ReaChR) linked via P2A to GCamp6f no Addgene #50954 Lin et al. 2013 and Chen et al. 2013
BA-101 pAAV-EF1a-Flex-ChR2(H134R)-YFP-MBD-WPRE EF1alpha Cre dependent expression of dentritic localized ChR2(H134R) fused to YFP and MBD (localized at dentrites (Myosin Binding Domain)) no Addgene #26973 and Addgene #21484 Lewis et al. 2009
BA-111 pAAV-mDlx-ChR2-mCherry-Fishell-3 mDLX ChR2-mCherry fusion expression in forebrain GABA-0ergic interneurons under the control of the mDlx enhancer element no Addgene #83898/ Dimidschstein et al. 2016

Optogenetische Inaktivatoren für Neurowissenschaften

Catalog # Vector name Promoter Discription available as "ready-made" AAV MTA informations Ref for publication
BA-004 pAAV-CamKII-NPHR3.0-eYFP CamKII Expression of halorhodopsin eNpHR3.0 enables optical control with far-red light no Addgene #26971 Gradinaru et al. 2010
BA-005 pAAV-CamKII-ArchT-GFP CamKII Expression of ArchT-GFP for neuronal silencing no Addgene #37807 Han et al. 2011
BA-011 pAAV-CAG-Flex-slowCloC-mcherry CAG Cre dependent expression of slowChloC fused to mCherry to inhibit neuronal acctivity. Subclone of addgene #85462 yes, Serotype AAV9 Addgene #85462 Wietek et al. 2014
BA-012 pAAV-CamKII-slowCloC-YFP CamKII Expression of slowChloC fused to YFP to inhibit neuronal acctivity. Subclone of addgene #85462 yes, Serotype AAV9 Addgene #85462 Wietek et al. 2014
BA-014 pAAV-CAG-Flex-slowCloC-YFP CAG Cre dependent expression of slowChloC fused to YFP to inhibit neuronal acctivity. Subclone of addgene #85462 no Addgene #85462 Wietek et al. 2014
BA-028 pAAV-Syn-Syp-miniSOG-T2A-mCherry hSynapsin1 Synaptophysin fused to miniSOG and linked via T2A to mCherry for optogenetic inhibition of synaptic release no Addgene #50972 Lin et al. 2013
BA-032 pAAV-CAG FLEX-Arch-GFP CAG Cre recombinase dependent Expression of Arch fused to GFP for silencing neuronal activity no Addgene #22222 Chow et al. 2010
BA-034 pAAV-CAG-Flex-Jaws-KGC-GFP-ER2 CAG Cre dependent expression of the red-shifted cruxhalorhodopsin Jaws fused to GFP no Addgene #84446 Chuong et al. 2014
BA-035 pAAV-hSyn-Jaws-KGC-GFP-ER2 hSynapsin1 Expression of the red-shifted cruxhalorhodopsin Jaws fused to GFP no Addgene #65014 Chuong et al. 2014
BA-036 pAAV-CamKII-Jaws-KGC-GFP-ER2 CamKII Expression of the red-shifted cruxhalorhodopsin Jaws fused to GFP no Addgene #65015 Chuong et al. 2014

Expression von intrazellulären Markern

Catalog # Vector name Promoter Discription available as "ready-made" AAV MTA informations Ref for publication
BA-023 pAAV-Syn-tdTomato-P2A-GFP-ER hSynapsin1 Expression of tdTomato linked via P2A to GFP-ER no - -

Markierung von Synapsen

Catalog # Vector name Promoter Discription available as "ready-made" AAV MTA informations Ref for publication
BA-020 paavCAG-pre-mGRASP-mCerulean-2A-nls-mCherry CAG For mapping synaptic connectivity: presynaptic mGRASP fused to mCerulean and linked via 2A to NLS-mcherry no Addgene #34911 Kim et al. 2011
BA-021 paavCAG-post-mGRASP-2A-dTomato CAG For mapping synaptic connectivity: postsynaptic mGRASP linked via 2A to dTomato no Addgene #34912 Kim et al. 2011
BA-022 paavCAG-Jx-rev-post-mGRASP-2A-dTomato CAG For mapping synaptic connectivity: Cre dependent expression of postsynaptic mGRASP linked via 2A to dTomato no Addgene #34913 Kim et al. 2011
BA-109 pAAV hSyn FLEX mGFP-2A-Synaptophysin-mRuby hSynapsin1 Cre dependent expression of membrane bound GFP linked via 2A to Synaptophysin-mRuby no Addgene #71760 Beier et al. 2015

Rekombinase Expressionsvektoren

Catalog # Vector name Promoter Discription available as "ready-made" AAV MTA informations Ref for publication
BA-009 pAAV-pgk-Cre PGK Cre rekombinase   Addgene #24593 -
BA-033 pAAV CAG CreNLS_eGFP CAG Nuclear targeted Cre rekombinase fused to GFP yes, Serotype AAV9, PHP.S - -
BA-048 pAAV-Syn-iCre-RFP hSynapsin1 Expression of codon inproved Cre rekombinase fused to RFP yes, Serotype AAV8, AAV9 - -

Generelle fluoreszierende Expressions Vektoren

Catalog # Vector name Promoter Discription available as "ready-made" AAV MTA informations Ref for publication
BA-001 pAAV-CAG-GFP CAG cytosolic GFP expression yes, Serotype AAV1, AAV6, PHP.S Addgene #37825 -
BA-002 pAAV-CAG-flex-GFP CAG Cre recombinase dependent GFP expression yes, Serotype AAV8, AAV9, PHP.S Addgene #28304 -
BA-010 pAAV-EF1a-floxed mCherry WPRE EF1alpha Cre dependet expression of mCherry no Addgene #20299 -
BA-015 pAAV-hSyn-NLS-GFP-P2A hSynapsin1 Expression of nuclear localised GFP fused to a P2A sequence yes, Serotype AAV9 - -
BA-016 pAAV-hSyn-NLS-RFP-P2A hSynapsin1 Expression of nuclear localised RFP fused to a P2A sequence yes, Serotype AAV8 - -
BA-017 pAAV-hSyn-CFP-P2A hSynapsin1 Expression of nuclear localised CFP fused to a P2A sequence no - -
BA-018 pAAV-hSyn-mkate2-P2A hSynapsin1 Expression of mkate2 fused to a P2A sequence no - -
BA-019 pAAV-hSyn-tdTomato-P2A hSynapsin1 Expression of tdTomato fused to a P2A sequence no - -
BA-024 pAAV-hSyn-mCherry-P2A hSynapsin1 Expression of mCherry fused to a P2A sequence yes, Serotype AAV6, AAV9 - -
BA-106 pAAV-CAG-tdtomato-WPRE CAG Expression of tdTomato no - -

Vektoren für den Einsatz von Rabies Viren

Catalog # Vector name Promoter Discription available as "ready-made" AAV MTA informations Ref for publication
BA-064 pAAV-EF1a-DIO-HTB EF1alpha Cre dependent expression of Histone tagged GFP linked via T2A to TVA receptor linked via E2A to Rabies B19G no Addgene #44187 -
BA-068 pAAV-Ef1a-DIO-H2B-GFP-2A-oG-WPRE-hGG EF1alpha Cre dependent expression of Histone tagged GFP linked via T2A to optimized Rabies B19G (oG) no Addgene #74289 Kim et al. 2016
BA-091 pAAV-Syn-H2B-GFP-TVA-oG-WPRE3 hSynapsin1, WPRE3 Enhanced Expression of Histone tagged GFP linked via T2A to TVA receptor linked via E2A to Rabies B19oG yes, Serotype AAV1 Addgene #74289 Kim et al. 2016
BA-096 pAAV-Syn-FLEX-nGToG-WPRE3 hSynapsin1 Enhanced Cre dependent expression of nuclear localized (NLS) GFP linked via T2A to TVA receptor linked via E2A to Rabies B19oG yes, Serotype AAV1 Addgene #74289 Kim et al. 2016