Katalog der AAV Vektoren und Viren

Hier finden Sie eine Übersicht zu unseren verschiedenen AAV Plasmid-Vektoren und bereits vorhandenen Viren (Ready-made). Sie können in unserem Bestellsystem die entsprechende Katalognummer eintragen und ihren gewünschten Virus produzieren lassen.

Externe Nutzer müssen einen MTA zwischen der Charité und ihrer Einrichtung abschließen und bei bei einigen Vektoren müssen entsprechende Addgene Regularien angewendet werden. Nähere Informationen erhalten Sie hierzu während des Bestellprozesses.

 

 

Sie befinden sich hier:

AAV Werkzeuge für Ihre Wissenschaft

Mit welchem Vektor/Virus können wir Ihnen helfen? Sortiert nach ihren spezifischen Transgenen, finden Sie hier verschiedenste Werkzeuge, die Ihnen in Ihrer wissenschaftlichen Fragestellung helfen könnte. Falls ein Vektor für Sie interessant ist, können Sie ihn als regular oder large Scale in unserem Ordersystem bestellen. Wenn der Vektor bereits als "ready-made" Virus in unseren Stocks existiert, können wir ihn für Pilot Studien schnell zur Verfügung stellen.

Wir versuchen die Liste immer aktuell zu halten. Kontaktieren sie uns bitte falls ihr gewünschter Vektor nicht dabei ist. Nutzen Sie unseren DNA Service falls wir vorhandene Vektoren für Sie spezifisch modifizieren könnten.

Optogenetische Sensoren für Neurowissenschaften

Catalog #

Vector name

Promoter

Discription

available as "ready-made" AAV

MTA informations

Ref for publication

BA-025

pAAV-hSyn-mCherry-P2A-GCaMP6f

hSynapsin1

Expression of mCherry linked via a P2A sequence to GCaMP6f, sublcone of addgene #40755

no

Addgene #40755

Chen et al. 2013

BA-026

pAAV Syn ruby-P2A-GCamp6f

hSynapsin1

Expression of Ruby linked via a P2A sequence to GCaMP6f

yes, Serotype AAV9

Addgene #50943

Rose et al. 2016

BA-027

pAAV Syn ruby-P2A-GCamp6s

hSynapsin1

Expression of Ruby linked via a P2A sequence to GCaMP6s

no

Addgene #50942

Rose et al. 2016

BA-037

pAAV-hSyn-CaMPARI-w

hSynapsin1

Expression of CaMPARI, a calcium-modulated photoactivatable ratiometric integrator. Subclone of addgene #60421

no

Addgene #60421

Fosque et al. 2015 and Zolnik et al. 2017

BA-038

pAAV-hSyn-Flex-CaMPARI-w

hSynapsin1

Cre-dependent expression of CaMPARI, a calcium-modulated photoactivatable ratiometric integrator.

yes, Serotype AAV2retro

Addgene #60421

Fosque et al. 2015 and Zolnik et al. 2017

BA-077

pAAV-Syn-flex-NES-jRCaMP1b-MBD-WPRE3

hSynapsin1

Cre dependent expression of jRCamP1b localized to dentrites (Myosin Binding Domain, MBD). Subclone of addgene #63136 and #21484)

yes, Serotype AAV9

Addgene #63136 and Addgene #21484

Dana et al. 2016 and Lewis et al. 2009

BA-079

pAAV-Syn-flex-NES-jRCaMP1b-WPRE3

hSynapsin1

Enhanced Cre dependent expression of the Red fluorescent calcium sensor protein jRCaMP1b, subclone of addgene #63136

yes, Serotype AAV9

Addgene #63136

Dana et al. 2016

BA-095

pAAV Syn Flex GCamp6f-wpre3

hSynapsin1

Cre-dependent expression of GCaMP6f

yes, Serotype AAV2retro

Addgene #40755

Chen et al. 2013

BA-102

pAAV-RAM-d2TTA::TRE-MCS-WPRE-pA

RAM/TRE

Robust activity marking system for exploring active neuronal ensembles. Empty vector, use in combination with DNA service request.

no

Addgene #63931

Sorensen et al. 2016

BA-103

pAAV-RAM-d2TTA::TRE-FLEX-MCS-WPRE-pA

RAM/TRE

Robust activity marking system for exploring active neuronal ensembles. Empty vector - Cre dependent, use in combination with DNA service request.

no

Addgene #84467

Sorensen et al. 2016

BA-104

pAAV-RAM-d2TTA::TRE-EGFP-WPREpA

RAM/TRE

Robust activity marking system for exploring active neuronal ensembles. GFP Expressing

no

Addgene #84469

Sorensen et al. 2016

BA-107

AAV-Syn-ER-GCamp6-150-WPRE3

hSynapsin1

Fluorescent reporter for ER calcium signaling - Expression of ER-GCaMP6-150

no

Addgene #86918

de Juan-Sanz et al. 2017

BA-108

AAV-Syn-ER-GCamp6-210-WPRE3

hSynapsin1

Fluorescent reporter for ER calcium signaling - Expression of ER-GCaMP6-210

no

Addgene #86919

de Juan-Sanz et al. 2017

BA-110

pAAV-mDlx-GCaMP6f-Fishell-2

mDLX

GCaMP6f expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

yes, Serotype AAV9, PHP.eB

Addgene #83899/

Dimidschstein et al. 2016

Optogenetische Aktivatoren für Neurowissenschaften

Catalog #

Vector name

Promoter

Discription

available as "ready-made" AAV

MTA informations

Ref for publication

BA-003

pAAV-Syn-flex ChR2(H134R)-YFP-WPRE

hSynapsin1

Cre recombinase dependent Expression of hChR2(H134R)-YFP, subclone of addgene #26973

yes, Serotype AAV1, AAV2retro

Addgene #26973

-

BA-006

pAAV-hSyn-hCHR2(H134R)-eYFP-WPRE

hSynapsin1

Expression of hChR2(H134R) fused to YFP for optical controling neuronal activation

yes, Serotype AAV1, AAV2retro

Addgene #26973

-

BA-007

pAAV-CAG-flex rev-Chr2wt-tdTomato -WPRE

CAG

Cre dependent expression of WT-hChR2 fused to tdTomato

no

Addgene #18917

Atasoy et al. 2008

BA-029

pACAGW-oChiEFtdTomato-AAV

CAG

Expression of oChIEF (chimera of ChR1 and ChR2) fused to tdTomato

no

Addgene #32846

Lin et al. 2009 and Jurgens et al. 2012

BA-030

pAAV-Flex-rev-oChIEF-tdTomato

CAG

Cre dependent expression of oChIEF (chimera of ChR1 and ChR2) fused to tdTomato

yes, Serotype AAV2

Addgene #30541

Lin et al. 2009 and Bell et al. 2011

BA-031

pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE

EF1alpha

Cre recombinase dependent Expression of hChR2(H134R) fused to YFP

no

Addgene #20298

-

BA-044

pAAV-CAG Flex-hChR2-T159C-P2A-eYFP

CAG

Cre dependent expression of Chr2 T159C linked via P2A to YFP, subclone of addgene #35511

no

Addgene #35511

Bernd et al. 2011 and Mattis et al. 2011

BA-049

pAAV CAG Flex hChR2(H134R)-mCherry-WPRE

CAG

Cre dependent expression of hChR2(H134R) fused to mCherry for optical controling neuronal activation

yes, Serotype AAV1, AAV6

Addgene #26973

-

BA-066

pAAV-CamKII-ReaChR-P2A-YFP

CamKII

Expression of a red-shifted variant of channelrhodopsin (ReaChR) linked via P2A to YFP

no

Addgene #50954

Lin et al. 2013

BA-074

pAAV-Syn-hChR2(H134R)-YFP-WPRE3

hSynapsin1, WPRE3

Enhanced expression of hChR2(H134R) fused to YFP for optical controling neuronal activation using a WPRE3 sequence, subclone of addgene #26973 and #61463

no

Addgene #26973 and Addgene #61463

Choi et al. 2014

BA-078

pAAV-CMV-hChR2(H134R)-YFP.WPRE3

CMV, WPRE3

Enhanced expression of hChR2(H134R) fused to YFP for optical controling neuronal activation using a WPRE3 sequence, subclone of addgene #26973 and #61463

no

Addgene #26973 and Addgene #61463

Choi et al. 2014

BA-080

pAAV-hSyn-ReaChR-p2A-mRuby

hSynapsin1

Expression of a red-shifted variant of channelrhodopsin (ReaChR) linked via P2A to mRuby

no

Addgene #50954

Lin et al. 2013

BA-081

pAAV-hSyn-ReaChR-p2A-tdTomato

hSynapsin1

Expression of a red-shifted variant of channelrhodopsin (ReaChR) linked via P2A to tdTomato

no

Addgene #50954 and für Addgene #40755

Lin et al. 2013

BA-084

pAAV-hSyn-ReaChR-p2A-gCamp6f

hSynapsin1

Expression of a red-shifted variant of channelrhodopsin (ReaChR) linked via P2A to GCamp6f

no

Addgene #50954

Lin et al. 2013 and Chen et al. 2013

BA-101

pAAV-EF1a-Flex-ChR2(H134R)-YFP-MBD-WPRE

EF1alpha

Cre dependent expression of dentritic localized ChR2(H134R) fused to YFP and MBD (localized at dentrites (Myosin Binding Domain))

no

Addgene #26973 and Addgene #21484

Lewis et al. 2009

BA-111

pAAV-mDlx-ChR2-mCherry-Fishell-3

mDLX

ChR2-mCherry fusion expression in forebrain GABA-0ergic interneurons under the control of the mDlx enhancer element

no

Addgene #83898/

Dimidschstein et al. 2016

Optogenetische Inaktivatoren für Neurowissenschaften

Catalog #

Vector name

Promoter

Discription

available as "ready-made" AAV

MTA informations

Ref for publication

BA-004

pAAV-CamKII-NPHR3.0-eYFP

CamKII

Expression of halorhodopsin eNpHR3.0 enables optical control with far-red light

no

Addgene #26971

Gradinaru et al. 2010

BA-005

pAAV-CamKII-ArchT-GFP

CamKII

Expression of ArchT-GFP for neuronal silencing

no

Addgene #37807

Han et al. 2011

BA-011

pAAV-CAG-Flex-slowCloC-mcherry

CAG

Cre dependent expression of slowChloC fused to mCherry to inhibit neuronal acctivity. Subclone of addgene #85462

yes, Serotype AAV9

Addgene #85462

Wietek et al. 2014

BA-012

pAAV-CamKII-slowCloC-YFP

CamKII

Expression of slowChloC fused to YFP to inhibit neuronal acctivity. Subclone of addgene #85462

yes, Serotype AAV9

Addgene #85462

Wietek et al. 2014

BA-014

pAAV-CAG-Flex-slowCloC-YFP

CAG

Cre dependent expression of slowChloC fused to YFP to inhibit neuronal acctivity. Subclone of addgene #85462

no

Addgene #85462

Wietek et al. 2014

BA-028

pAAV-Syn-Syp-miniSOG-T2A-mCherry

hSynapsin1

Synaptophysin fused to miniSOG and linked via T2A to mCherry for optogenetic inhibition of synaptic release

no

Addgene #50972

Lin et al. 2013

BA-032

pAAV-CAG FLEX-Arch-GFP

CAG

Cre recombinase dependent Expression of Arch fused to GFP for silencing neuronal activity

no

Addgene #22222

Chow et al. 2010

BA-034

pAAV-CAG-Flex-Jaws-KGC-GFP-ER2

CAG

Cre dependent expression of the red-shifted cruxhalorhodopsin Jaws fused to GFP

no

Addgene #84446

Chuong et al. 2014

BA-035

pAAV-hSyn-Jaws-KGC-GFP-ER2

hSynapsin1

Expression of the red-shifted cruxhalorhodopsin Jaws fused to GFP

no

Addgene #65014

Chuong et al. 2014

BA-036

pAAV-CamKII-Jaws-KGC-GFP-ER2

CamKII

Expression of the red-shifted cruxhalorhodopsin Jaws fused to GFP

no

Addgene #65015

Chuong et al. 2014

Expression von intrazellulären Markern

Catalog #

Vector name

Promoter

Discription

available as "ready-made" AAV

MTA informations

Ref for publication

BA-023

pAAV-Syn-tdTomato-P2A-GFP-ER

hSynapsin1

Expression of tdTomato linked via P2A to GFP-ER

no

-

-

Markierung von Synapsen

Catalog #

Vector name

Promoter

Discription

available as "ready-made" AAV

MTA informations

Ref for publication

BA-020

paavCAG-pre-mGRASP-mCerulean-2A-nls-mCherry

CAG

For mapping synaptic connectivity: presynaptic mGRASP fused to mCerulean and linked via 2A to NLS-mcherry

no

Addgene #34911

Kim et al. 2011

BA-021

paavCAG-post-mGRASP-2A-dTomato

CAG

For mapping synaptic connectivity: postsynaptic mGRASP linked via 2A to dTomato

no

Addgene #34912

Kim et al. 2011

BA-022

paavCAG-Jx-rev-post-mGRASP-2A-dTomato

CAG

For mapping synaptic connectivity: Cre dependent expression of postsynaptic mGRASP linked via 2A to dTomato

no

Addgene #34913

Kim et al. 2011

BA-109

pAAV hSyn FLEX mGFP-2A-Synaptophysin-mRuby

hSynapsin1

Cre dependent expression of membrane bound GFP linked via 2A to Synaptophysin-mRuby

no

Addgene #71760

Beier et al. 2015

Rekombinase Expressionsvektoren

Catalog #

Vector name

Promoter

Discription

available as "ready-made" AAV

MTA informations

Ref for publication

BA-009

pAAV-pgk-Cre

PGK

Cre rekombinase

 

Addgene #24593

-

BA-033

pAAV CAG CreNLS_eGFP

CAG

Nuclear targeted Cre rekombinase fused to GFP

yes, Serotype AAV9, PHP.S

-

-

BA-048

pAAV-Syn-iCre-RFP

hSynapsin1

Expression of codon inproved Cre rekombinase fused to RFP

yes, Serotype AAV8, AAV9

-

-

Generelle fluoreszierende Expressions Vektoren

Catalog #

Vector name

Promoter

Discription

available as "ready-made" AAV

MTA informations

Ref for publication

BA-001

pAAV-CAG-GFP

CAG

cytosolic GFP expression

yes, Serotype AAV1, AAV6, PHP.S

Addgene #37825

-

BA-002

pAAV-CAG-flex-GFP

CAG

Cre recombinase dependent GFP expression

yes, Serotype AAV8, AAV9, PHP.S

Addgene #28304

-

BA-010

pAAV-EF1a-floxed mCherry WPRE

EF1alpha

Cre dependet expression of mCherry

no

Addgene #20299

-

BA-015

pAAV-hSyn-NLS-GFP-P2A

hSynapsin1

Expression of nuclear localised GFP fused to a P2A sequence

yes, Serotype AAV9

-

-

BA-016

pAAV-hSyn-NLS-RFP-P2A

hSynapsin1

Expression of nuclear localised RFP fused to a P2A sequence

yes, Serotype AAV8

-

-

BA-017

pAAV-hSyn-CFP-P2A

hSynapsin1

Expression of nuclear localised CFP fused to a P2A sequence

no

-

-

BA-018

pAAV-hSyn-mkate2-P2A

hSynapsin1

Expression of mkate2 fused to a P2A sequence

no

-

-

BA-019

pAAV-hSyn-tdTomato-P2A

hSynapsin1

Expression of tdTomato fused to a P2A sequence

no

-

-

BA-024

pAAV-hSyn-mCherry-P2A

hSynapsin1

Expression of mCherry fused to a P2A sequence

yes, Serotype AAV6, AAV9

-

-

BA-106

pAAV-CAG-tdtomato-WPRE

CAG

Expression of tdTomato

no

-

-

Vektoren für den Einsatz von Rabies Viren

Catalog #

Vector name

Promoter

Discription

available as "ready-made" AAV

MTA informations

Ref for publication

BA-064

pAAV-EF1a-DIO-HTB

EF1alpha

Cre dependent expression of Histone tagged GFP linked via T2A to TVA receptor linked via E2A to Rabies B19G

no

Addgene #44187

-

BA-068

pAAV-Ef1a-DIO-H2B-GFP-2A-oG-WPRE-hGG

EF1alpha

Cre dependent expression of Histone tagged GFP linked via T2A to optimized Rabies B19G (oG)

no

Addgene #74289

Kim et al. 2016

BA-091

pAAV-Syn-H2B-GFP-TVA-oG-WPRE3

hSynapsin1, WPRE3

Enhanced Expression of Histone tagged GFP linked via T2A to TVA receptor linked via E2A to Rabies B19oG

yes, Serotype AAV1

Addgene #74289

Kim et al. 2016

BA-096

pAAV-Syn-FLEX-nGToG-WPRE3

hSynapsin1

Enhanced Cre dependent expression of nuclear localized (NLS) GFP linked via T2A to TVA receptor linked via E2A to Rabies B19oG

yes, Serotype AAV1

Addgene #74289

Kim et al. 2016