Katalog der Lentiviralen Vektoren

BL-0700

f(syn)-Syp-GCamp6f-w

hSynapsin1

Synaptophysin,GCamp6f

Synaptophysin fused C terminally with GCamp6f (subclone of addgene #40755)

Addgene #40755

Chen et al. 2013

BL-0570

f(syn)-NLS.GFP-P2A-GCamp6f-w

hSynapsin1

GCamp6f

Nuclear GFP linked via P2A to GCamp6f (Subclone of addgene #40755)

Addgene #40755

Chen et al. 2013

BL-1231

f(syn)-NLS.CFP-P2A-Syp-GCamp6f

hSynapsin1

Synaptophysin, GCamP6f, NLS.CFP

Nuclear CFP linked via P2A to GCamp6f (Subclone of addgene #40755)

Addgene #40755

Chen et al. 2013

BL-1331

f(syn)SWITCH(Syp-GCamp6f)

hSynapsin1

Synaptophysin,GCamp6f

Cre-ON: Synaptophysin fused C terminally with GCamp6f (subclone of addgene #40755)

Addgene #40755

Chen et al. 2013

BL-1338

f(syn)-Flox(Syp-GCamp6f)

hSynapsin1

Synaptophysin,GCamp6f

Cre-OFF: Synaptophysin fused C terminally with GCamp6f (subclone of addgene #40755)

Addgene #40755

Chen et al. 2013

BL-1329

f(syn)-Syp-jRCaMP1b

hSynapsin1

Synaptophysin, jRCaMP1b

Synaptophysin fused C terminally with jRCaMP1b (subclone of addgene #63136)

Addgene #63136

Dana et al. 2016

BL-1505

f(syn)-NES-jRCaMP1b-WPRE

hSynapsin1

NES-jRCaMP1b

Subclone of addgene #63136

Addgene #63136

Dana et al. 2016

BL-1471

pLEX-R_GECO1

hPGK

R-GECO1

Expression of R-GECO1.0 under the control of the hPGK Promoter. Subclone of Addgene #32444 in pLex_305 (Addgene #41390)

Addgene #32444 and Addgene #41390

Zhao et al. 2011

Catalog #

Vector name

Promoter

Gene of Interest

Discription

MTA informations

Ref for publication

BL-0047

f(syn)-SypHy-w

hSynapsin1

Synaptophysin, pHluorin

Expression of Rat Synaptophysin-pHluorin, subclone of addgene #24478

Addgene #24478

Granseth et al. 2006

BL-0099

f(syn)-Syb2-pH

hSynapsin1

Synaptpbrevin2 (Vamp2), pHluorin

Synaptobrevin2 (Vamp2) fused c-termially to pHluorin, subclone of addgene #24478

Addgene #24478

Granseth et al. 2006

BL-0664

f(syn)Switch(revSyphy)

hSynapsin1

Synaptophysin, pHluorin

Cre-On expression of Rat Synaptophysin-pHluorin, subclone of addgene #24478

Addgene #24478

Herman et al. 2018

BL-0676

f(syn)Flox(Syphy)

hSynapsin1

Synaptophysin, pHluorin

Cre-off: expression of Rat Synaptophysin-pHluorin in non-Cre expressing cells, subclone of addgene #24478

Addgene #24478

Herman et al. 2018

BL-0647

f(syn)SWITCH(RFP_revSypHy)w

hSynapsin1

Synaptophysin, pHluorin

CreON/OFF Vector: Expression of RFP in Non-Cre cells, and of Rat Synaptophysin-pHluorin in Cre cells. Subclone of addgene #24478

Addgene #24478

Granseth et al. 2006

HL-0121

f(syn)-VGlut1pH-w

hSynapsin1

VGlut1 , pHluorin

VGlut1 fused to pHLuorin in within the luminal part of the Transporter

-

Herman et al. 2014

BL-0247

f(syn)-VGlut2pH-w

hSynapsin1

VGlut2 , pHluorin

VGlut2 fused to pHLuorin in within the luminal part of the Transporter

-

Herman et al. 2014

BL-0336

f(syn)-Phönix-w

hSynapsin1

Halorhodopsin, SypHy and mkate2

Halorhodopsin fused to SypHy and mkate2 (synaptic vesicle proton pump and pH sensor)

Addgene #70111

Rost et al. 2016

BL-0344

f(syn)-Syt1-pHluorin-w

hSynapsin1

Synaptotagmin1, pHluorin

Synaptotagmin1 fused N terminally with pHLuorin, subclone of addgene #24478

Addgene #24478

-

BL-0553

f(syn)-Syp-pH-mkate2-w

hSynapsin1

Synaptophysin, luminal pHluorin

Synaptophysin with Luminal pHLuorin and cytosolic mkate2

-

-

BL-0987

f(syn)-NLS.RFP-P2A-SEP-GluR1

hSynapsin1

GluR1, pHluorin

Expression of nuclear RFP linked via P2A to GluR1 fused to super-ecliptic pHlourin, subclone of addgene# 24000

Addgene #24000

Kopec et al. 2006

BL-0988

f(syn)-NLS.RFP-P2A-SEP-GluR2

hSynapsin1

GluR2, pHluorin

Expression of nuclear RFP linked via P2A to GluR2 fused to super-ecliptic pHlourin, subclone of addgene# 24001

Addgene #24001

Kopec et al. 2006

BL-1849

f(syn)syphy-tdimer2

hSynapsin1

Synaptophysin, pHluorin

Expression of Synaptophysin-pHluorin and tdimer2 for ratiometric imaging. Subclone of addgene #44268.

Addgene #44268

Rose et al. 2013

BL-1363

f(syn)GRAB-DA1h

hSynapsin1

GRAB-DA1h

Expression of the fluorescent dopamine(DA) sensor GRAB_DA1h in neurons. Subclone of addgene #113050

Addgene #113050

Sun et al. 2018

BL-1364

f(syn)GRAB-DA1m

hSynapsin1

GRAB-DA1h

Expression of the fluorescent dopamine(DA) sensor GRAB_DA1m in neurons. Subclone of addgene #113049

Addgene #113049

Sun et al. 2018

BL-1366

f(syn)dLight1.1

hSynapsin1

dLight1.1

Expression of the dopamine(DA) indicator dLight1 in neurons. Subclone of addgene #111053

Addgene #111053

Patriarchi et al. 2018

BL-1843

f(syn)Switch(SynATP)-w

hSynapsin1

SynATP

Cre-dependent expression (CreON) of an optical reporter of presynaptic ATP. Subclone of addgene #111053

Addgene #51819

Rangaraju et al. 2014

BL-1844

f(syn)Flox(SynATP)-w

hSynapsin1

SynATP

Cre-dependent expression (CreOFF) of an optical reporter of presynaptic ATP. Subclone of addgene #111053

Addgene #51819

Rangaraju et al. 2014

BL-0336

f(syn)-Phönix-w

hSynapsin1

Halorhodopsin, SypHy and mkate2

Halorhodopsin fused to SypHy and mkate2 (synaptic vesicle proton pump and pH sensor)

Addgene #70111

Rost et al. 2016

BL-0347

f(syn)-ChR2-E123T/T159C-YFP-w

hSynapsin1

ChR2 (ChetaTC)

ChR2 (ChetaTC) fused to YFP. Subclone of addgene #35511

Addgene #35511

Bernd et al. 2011 and Mattis et al. 2011

BL-0365

f(syn)-CreON(ChR2-E123T/T159C-YFP)-w

hSynapsin1

ChR2 (ChetaTC)

CreON Vector: ChR2 (ChetaTC) fused to YFP. Subclone of addgene #35511

Addgene #35511

Bernd et al. 2011 and Mattis et al. 2011

BL-0370

f(syn)-SWITCH(RFP_revChR2-E123T/T159C-YFP)-w

hSynapsin1

ChR2 (ChetaTC

CreON/OFF Vector: Expression of RFP in Non-Cre cells, Expression of ChR2 (ChetaTC) in Cre cells. Subclone of addgene #35511

Addgene #35511

Bernd et al. 2011 and Mattis et al. 2011

BL-0644

f(syn)-CreON(NLS.RFP-P2A-ChR2-ET/TC)-w

hSynapsin1

ChR2 (ChetaTC

CreOn Vector: NLS.RFP linked to fast ChR2 (ChetaTC) via P2A, Cre dependent expression, subclone of addgene #35511

Addgene #35511

Bernd et al. 2011 and Mattis et al. 2011

BL-0662

f(syn)-CreOFF(ChR2-E123T/T159C-YFP)-w

hSynapsin1

ChR2 (ChetaTC

CreOFF Vector: Expression of ChR2 (ChetaTC) in Non-Cre cells. Subclone of addgene #35511

Addgene #35511

Bernd et al. 2011 and Mattis et al. 2011

BL-0679

f(syn)-ChR2-H134R-YFP-w

hSynapsin1

Chr2-H134R

Expression of Chr2-H134R fused to YFP, subclone of addgene #26973

Addgene #26973

-

BL-0693

f(syn)-ChR2-E123T/T159C-YFP-P2A-MCS-w

hSynapsin1

ChR2 (ChetaTC)

ChR2 (ChetaTC) fused to YFP and linked via a P2A sequence to a multiple cloning site (MSC). Subclone of addgene #35511

Addgene #35511

Bernd et al. 2011 and Mattis et al. 2011

BL-0792

f(syn)-VChR1-eYFP-w

hSynapsin1

ChR1

Expression of Red-shifted volvox ChR1 fused to YFP, subclone of addgene #20955

Addgene #20955

Zhang et al. 2008

BL-0903

f(CamKII)-ChrimsonR-GFP-w

CamKII

ChrimsonR

Expression of ChrimsonR fused to GFP

Addgene #59049

Klapoetke et al. 2014

BL-1847

f(syn)ChR2-ET/TC-mscarlet

hSynapsin1

ChR2 (ChetaTC)

ChR2 (ChetaTC) fused to mScarlet. Subclone of addgene #35511

Addgene #35511

Bernd et al. 2011 and Mattis et al. 2011

BL-1848

f(syn)ChR2-ET/TC-Cerulean3

hSynapsin1

ChR2 (ChetaTC)

ChR2 (ChetaTC) fused to Cerulean3. Subclone of addgene #35511

Addgene #35511

Bernd et al. 2011 and Mattis et al. 2011

BL-0735

f(syn)-mCherry-Cry2PHR-w

hSynapsin1

Cryptochrome2

Expression of mCherry tagged Cryptochrome2 (aa1-498), subclone from addgene #28244

Addgene #28244

Kennedy et al. 2010

BL-0726

f(syn)-CIBN-GFP-TM-Stx1a-w

hSynapsin1

CIB1

Expression of a truncated version of a basic helix-loop-helix protein Arabidopsis CIB1 (1-170) fused to GFP and the transmembrane domain of Syntaxin1a, subclone of addgene #28246

Addgene #28246

Kennedy et al. 2010

BL-0732

f(syn)-CIBN-TM-Stx1a-w

hSynapsin1

CIB1

Expression of a truncated version of a basic helix-loop-helix protein Arabidopsis CIB1 fused to the transmembrane domain of Syntaxin1a, subclone of addgene #28246

Addgene #28246

Kennedy et al. 2010

BL-0802

f(syn)-mCherry-Cry2PHR-P2A-MCS-w

hSynapsin1

Cryptochrome2

Expression of mCherry tagged Cryptochrome2 (aa1-498) linked to a MCS via P2A, subclone from addgene #28244

Addgene #28244

Kennedy et al. 2010

BL-1142

f(syn)nMagHigh-eGFP-CAAX

hSynapsin1

VIVID

Blue light dependent photoswitching system: membrane bound nMag-eGFP. Subclone of addgene #67300

Addgene #67300

Kawano et al. 2015

BL-1136

f(syn)pMagFast2(3x)-iRPF

hSynapsin1

VIVID

Blue light dependent photoswitching system: soluble pMag-iRFP. Subclone of addgene #67297

Addgene #67297

Kawano et al. 2015

BL-1312

f(syn)CRY2PHR-mCherryN1

hSynapsin1

Cryptochrome2

Expression of the photolyase homology region (PHR) of cryptochrome 2 (Cry2, aa1-498) fused c-terminally to mCherry under control of the human synapsin1 promoter. Subclone of addgene #26866.

Addgene #26866

Kennedy et al. 2010

BL-1315

f(syn)CIBN(deltaNLS)-pmGFP

hSynapsin1

CIB1

Expression of the helix-loop-helix protein Arabidopsis CIB1 protein (aa1-170 with mutation of predicted NLS.Sequence fused to plasma targeted GFP (CAAX). Subclone of addgene #26867

Addgene #26867

Kennedy et al. 2010

BL-1313

f(syn)CRY2olig-mCherry

f(syn)

Cryptochrome2-Olig

Expression of the photolyase homology region (PHR) of cryptochrome 2 (Cry2, aa1-498 with E490G mutation (olig)) fused c-terminally to mCherry under control of the human synapsin1 promoter. Subclone of addgene #60032.

Addgene #60032

Taslimi et al. 2016

BL-1314

f(syn)CRY2PHR(W349R)-mCherry

f(syn)

CRY2PHR(W349R)

Expression of the photolyase homology region (PHR) of cryptochrome 2 (Cry2, aa1-498 with W349R mutation (fast-cycling)) fused c-terminally to mCherry under control of the human synapsin1 promoter. Subclone of addgene #75370.

Addgene #75370

Taslimi et al. 2016

BL-0100

f(syn)-GFP-P2A-MSCw

hSynapsin1

GFP

cytosolic GFP expression restricted to neurons (fused to a P2A sequence)

-

-

BL-0110

f(syn)-mkate2-P2A-MSCw

hSynapsin1

mkate2

mkate2 expression restricted to neurons (fused to a P2A sequence)

-

-

BL-0140

f(syn)-NLS.GFP-P2A-MSCw

hSynapsin1

NLS.GFP

nulcear localized GFP expression restricted to neurons (fused to a P2A sequence)

-

-

BL-0181

f(syn)-NLS.RFP-P2A-MSCw

hSynapsin1

NLS.RFP

nulcear localized RFP expression restricted to neurons (fused to a P2A sequence)

-

-

BL-0512

f(syn)-tdTomato-P2A-MSC

hSynapsin1

tdtomato

tdtomato expression restricted to neurons (fused to a P2A sequence)

-

-

BL-0526

f(CMV)-RFPw

CMV

RFP

CMV promoter controlled cytosolic RFP expression

-

-

BL-0533

f(CMV)-GFPw

CMV

GFP

CMV promoter controlled cytosolic GFP expression

-

-

BL-0534

f(CMV)-NLS.GFP-P2A-MSCw

CMV

NLS.GFP

CMV promoter controlled nuclear localized GFP expression (fused to a P2A sequence)

-

-

BL-0535

f(CMV)-NLS.RFP-P2A-MSCw

CMV

NLS.RFP

CMV promoter controlled nuclear localized RFP expression (fused to a P2A sequence)

-

-

BL-0540

f(GFAP2.2)-GFP-w

GFAP2.2

GFP

cytosolic GFP expression restricted in astrocytes

-

-

BL-0541

f(GFAP2.2)-NLS.GFP-P2A-MSCw

GFAP2.2

NLS.GFP

nuclear localized GFP expression restricted to astrocytes (fused to a P2A sequence)

-

-

BL-0542

f(GFAP2.2)-NLS.RFP-P2A-MSCw

GFAP2.2

NLS.RFP

nuclear localized RFP expression restricted to astrocytes (fused to a P2A sequence)

-

-

BL-0543

f(ubi)-NLS.GFP-P2A-MSCw

Ubiquitin

NLS.GFP

nuclear localized GFP expression (fused to a P2A sequence)

-

-

BL-0544

f(ubi)-NLS.RFP-P2A-MSCw

Ubiquitin

NLS.RFP

nuclear localized RFP expression (fused to a P2A sequence)

-

-

BL-0752

f(syn)-CFP-P2A-MSC

hSynapsin1

CFP

Cerulean expression (fused to a P2A sequence)

-

-

BL-1109

f(CAG)-GFP-w

CAG

GFP

CAG Promoter for cytosolic GFP expression

-

-

BL-1161

f(CAG)-NLS.GFP-w

CAG

NLS.GFP

CAG Promoter for nucklear localized GFP expression

-

-

BL-1181

f(syn)-NLS.CFP-P2A-w

hSynapsin1

NLS.CFP

nulcear localized CFP expression restricted to neurons (fused to a P2A sequence)

-

-

HL-0001

f(Ubi)-GFP-w

Ubiquitin

GFP

GFP expression under control of the Ubiquitin promoter (FUGW)

BL-1260

f(THP2.5)GFP-P2A-w

Tyrosine hydroxylse promoter (THP2.5)

GFP

GFP expression under control of the rat tyrosin hydroxylase promoter (2.5kb upstream, THP). Subclone of addgene clone #80336.

Addgene #80336

Oh et al. 2009

BL-1264

f(syn)-mKate-P2A-NLS.GFP

hSynapsin1

mkate2, NLS.GFP

Expression of mkate2 and NLS.GFP under the control of the human Synaptophysin1 promoter

-

-

BL-1388

CAG-NLS.RFP-P2A

CAG

NLS.RFP

CAG Promoter controlled nuclear localized RFP expression (fused to a P2A sequence)

-

-

BL-1643

f(CAG)-RFP-w

CAG

RFP

CAG Promoter controlled RFP expression

-

-

BL-1792

f(CMV)-RFP-P2A-MCS WPRE

CMV

RFP

CMV Promoter controlled RFP expression fused to a P2A and multiple cloning site.

-

-

BL-0443

f(syn)mkate2-P2A-GFP-ER

hSynapsin1

mkate2, GFPer

Far red mkate2 linked via P2A to endosomal reticulum bound GFP

-

-

BL-0452

f(syn)eGFP-ER

hSynapsin1

GFPer

Endosomal reticulum bound GFP

-

-

BL-0500

f(syn)Gap-GFP-P2A

hSynapsin1

GAP-GFP

Membran bound GFP due to N-terminal fused dual palmitoylation sequence of GAP43

-

-

BL-0515

f(syn)tdtomato-P2A-GFP-ER

hSynapsin1

tdtomato, GFPer

tdtomato linked via P2A to endosomal reticulum bound GFP

-

-

BL-0569

f(syn)Tom20-GFP

hSynapsin1

Tom20

Mitochondrial Tom20 fused to GFP

-

-

BL-0625

f(syn)Rab5a-GFP-w

hSynapsin1

Rab5a

Mouse Rab5a fused to GFP

-

-

BL-0721

f(syn)w-PA-GFP-Actin

hSynapsin1

ß-Actin

Photoactivatable GFP variant fused to Actin, subclone from addgene plasmid 57120

Addgene #57210

Olenych et al. 2007

BL-0722

f(syn)Lifeact-mEOS3.2

hSynapsin1

lifeact

Lifeact-mEOS3.2 expression, subclone from addgene plasmid 54696 into LV vector.

Addgene #54696

-

BL-0758

f(syn)w-eGFP-2FYFE

hSynapsin1

FYFE

two FYFE domains fused to GFP (PI3P binder)

Addgene #36096

Burd et al. 1998

BL-0855

f(syn)LifeAct-GFP-w

hSynapsin1

lifeact

Lifeact-GFP expression, subclone from addgene plasmid 54696 into LV vector.

Addgene #54696

BL-1250

f(syn)LifeAct-mkate2-w

hSynapsin1

lifeact

Lifeact sequence fused to mkate2, subclone from addgene plasmid 54696 into LV vector.

Addgene #54696

BL-1282

f(syn)eGFP-Rab8-w

hSynapsin1

Rab8

Rab8 fused to GFP, subclone of addgene #31803.

Addgene #31803

Guizetti et al. 2011

BL-1467

pLEX-CEPIA1er

hPGK

CEPIA1er

Expression of ER-tagged CEPIA1 under the control of the hPGK Promoter. Subclone of Addgene #58216 in pLex_305 (Addgene #41390)

Addgene #58216 and Addgene #41390

Suzuki et al. 2014

BL-1469

pLEX-CEPIA3mt

hPGK

CEPIA3mt

Expression of MT-tagged CEPIA3 under the control of the hPGK Promoter. Subclone of Addgene #58219 in pLex_305 (Addgene #41390)

Addgene #58219 and Addgene #41390

Suzuki et al. 2014

BL-1468

pLEX-CEPIA2mt

hPGK

CEPIA2mt

Expression of MT-tagged CEPIA2 under the control of the hPGK Promoter. Subclone of Addgene #58218 in pLex_305 (Addgene #41390)

Addgene #58218 and Addgene #41390

Suzuki et al. 2014

BL-1470

pLEX-CEPIA4mt

hPGK

CEPIA4mt

Expression of MT-tagged CEPIA4 under the control of the hPGK Promoter. Subclone of Addgene #58220 in pLex_305 (Addgene #41390)

Addgene #58220 and Addgene #41390

Suzuki et al. 2014

BL-1738

f(syn)Flag-Cortactin

hSynapsin1

Cortactin

Expression of the actin binding protein Cortactin fused to Flag Tag. Subclone of Addgene #74476.

Addgene #74476

Weed et al. 2000

BL-1746

f(syn)emerald-Cortactin

hSynapsin1

Cortactin

Expression of the actin binding protein Cortactin fused to emerald. Subclone of Addgene #54051.

Addgene #54051

-

BL-0141

f(syn)-NLS.GFP-P2A-VGlut1mkate2

hSynapsin1

Vglut1

Nuclear GFP linked via P2A to VGlut1 fused to mkate2

-

-

BL-0177

f(syn)-Syp-GFP-w

hSynapsin1

Synaptophysin

Synaptophysin fused to GFP

-

Sampathkumar et al. 2016

BL-0195

f(syn)-Syp-GFP-P2A-MCS-w

hSynapsin1

Synaptophysin

Synaptophysin fused to GFP and a P2A Sequence

-

Sampathkumar et al. 2016

BL-0258

f(syn)-NLS.GFP-P2A-Syp-mkate2

hSynapsin1

Synaptophysin

Nuclear GFP linked via P2A to Synaptophysin fused to mkate2

-

Sampathkumar et al. 2016

BL-0373

f(syn)-Syp-mkate2-w

hSynapsin1

Synaptophysin

Synaptophysin fused to mkate2

-

Sampathkumar et al. 2016

BL-0418

f(syn)-VGlut1-mkate2-w

hSynapsin1

VGlut1

VGlut1 fused to mkate2

-

-

BL-0492

f(syn)-VGlut1-GFP-w

hSynapsin1

VGlut1

VGlut1 fused to GFP

-

-

BL-0760

f(syn)-NLS.RFP-P2A-Syp-CFP-T2A-MCS

hSynapsin1

Synaptophysin

Nuclear RFP linked via P2A to Synaptophysin fused to CFP and linked to a second 2A site with a Multiple Cloning Site

-

-

BL-0856

f(syn)-LifeAct-GFP-P2A-Syp-mkate2

hSynapsin1

lifeact, Synaptophysin

Expression of Lifeact-GFP linked via P2A to Synaptophysin fused to mkate2, subclone from addgene #54696

Modifiction of Addgene #54696

-

BL-1033

f(syn)-Homer1-GFP-w

hSynapsin1

Homer1

Expression of postsynaptic Homer fused to GFP, subclone of addgene #57461

Addgene #57461

-

BL-1034

f(syn)-Homer1-tdtomato

hSynapsin1

Homer1

Expression of postsynaptic Homer fused to tdtomato, subclone of addgene #57461

Addgene #57461

-

BL-1035

f(syn)-Homer1-mEOS3.2-w

hSynapsin1

Homer1

Expression of postsynaptic Homer fused to mEOS3.2, , subclone of addgene #57461

Addgene #57461

-

BL-1038

f(syn)-GluR1-mEOS3.2

hSynapsin1

GluR1

Expression of postsynaptic GluR1 labeled with extracellulär mEOS3.2, subclone of addgene #57461

Addgene #57461

-

BL-1054

f(syn)-GluR2-mEOS3.2

hSynapsin1

GluR2

Expression of postsynaptic GluR2 labeled with extracellulär mEOS3.2, subclone of addgene #57461

Addgene #57461

-

BL-1122

f(syn)-Homer1-CFP-w

hSynapsin1

Homer1

Expression of postsynaptic Homer fused to CFP, , subclone of addgene #57461

Addgene #57461

-

BL-1823

f(syn)GFP-P2A_Sypmkate2

hSynapsin1

Synaptophysin

Expression of Synapotphysin fused to mkate2 and cytosolic GFP.

-

-

BL-0592

f(syn)-post-mGRASP-2A-dTomato-w

hSynapsin1

post-mGRASP

For mapping synaptic connectivity: postsynaptic mGRASP linked via 2A to dTomato (subclone from addgene clone #34912)

Addgene #34912

Kim et al. 2011

BL-0593

f(syn)-pre-mGRASP-mCerulean-2A-NLS.mCherry-w

hSynapsin1

pre-mGRASP

For mapping synaptic connectivity: presynaptic mGRASP fused to mCerulean and linked via 2A to NLS.mcherry (subclone from addgene clone #34911)

Addgene #34911

Kim et al. 2011

BL-0638

f(syn)-NLS.RFP-P2A-post-mGRASP-w

hSynapsin1

post-mGRASP

For mapping synaptic connectivity: Nuclear RFP linked via P2A with postsynaptic mGRASP (subclone from addgene clone #34912)

Addgene #34912

Kim et al. 2011

BL-0639

f(syn)-NLS.GFP-P2A-pre-mGRASP-mkate2-w

hSynapsin1

pre-mGRASP

For mapping synaptic connectivity: Nuclear GFP linked via P2A with presynaptic mGRASP fused to mkate2 (subclone from addgene clone #34911)

Addgene #34911

Kim et al. 2011

BL-0725

f(syn)w iRFP-PH (PI4,5P2)

hSynapsin1

PHD - PLCdelta1

PI4,5P2 marker: PH domain of human PLCdelta1 fused to iRFP

Addgene #36075 und Addgene #54786

Chisari et al., 2009 and Filinov et al., 2011

BL-1446

f(syn)PLCd(PHD)-eGFP-P2A.WPRE (PI4,5P2)

hSynapsin1

PHD - PLCdelta1

PI4,5P2 marker: PH domain of human PLCdelta1 fused to eGFP-P2A (subclone from addgene clone #51407)

Addgene #51407

P. Varnai and T. Balla, 1998

BL-1493

f(syn)PLCd(PHD)-mCherry-P2A.WPRE (PI4,5P2)

hSynapsin1

PHD - PLCdelta1

PI4,5P2 marker: PH domain of human PLCdelta1 (against PIP4,5) fused to mCherry-P2A (subclone from addgene clone #51407)

Addgene #51407

P. Varnai and T. Balla, 1998

BL-1453

f(Syn)GFP-GRP1(PHD)-w

hSynapsin1

PHD -GRP1

PIP3 marker: PH domain of GRP1 fused to GFP. Subclone of addgene clone #71378

Addgene #71378

Kavran et al., 1998

BL-1447

f(syn)Akt.PHD-RFP-w (PIP3)

hSynapsin1

PHD - AKT

PIP3 marker: PH domain of AKT fused to RFP. Subclone of addgene clone #51465

P. Varnai and T. Balla, 1998

BL-1450

f(syn)mcherry-DrrA-w

hSynapsin1

DrrA / SidM

Biosensor for PtdIns4P fused to mCherry. Subclone of addgene clone #51471

Hammond et al., 2014

BL-1431

f(syn)D4H-mCherry-w (Cholesterol)

hSynapsin1

D4H

A cholesterol biosensor, termed D4H, based on the fourth domain of Clostridium perfringens theta-toxin. Fusion with mCherry. Subclone into f(syn)w.

D4H-mCherry-w was a gift from Greg Fairn.

Maekawa & Fairn, 2015

BL-1492

f(syn)D4H-GFP-w (Cholesterol)

hSynapsin1

D4H

A cholesterol biosensor, termed D4H, based on the fourth domain of Clostridium perfringens theta-toxin. Fusion with GFP. Subclone of BL-1431.

D4H-mCherry-w was a gift from Greg Fairn.

Maekawa & Fairn, 2015

BL-0150

f(syn)iCreRFP-P2A-MSCw

hSynapsin1

iCre-RFP

Synapsin promoter dependent expression of Cre recombinase fused to a RFP-P2A sequence

-

Vardar et al. 2016

BL-0224

f(syn)iCreCFP-P2A-MSCw

hSynapsin1

iCre-CFP

Synapsin promoter dependent expression of Cre recombinase fused to a CFP-P2A sequence

-

-

BL-0300

f(syn)iCre-P2A-MSCw

hSynapsin1

iCre

Synapsin promoter dependent expression of Cre recombinase fused to a P2A sequence

-

-

BL-0366

f(syn)iCreGFP-P2A-MSCw

hSynapsin1

iCre-GFP

Synapsin promoter dependent expression of Cre recombinase fused to a GFP-P2A sequence

-

-

BL-0445

f(syn)FLP-GFP-P2A-MSCw

hSynapsin1

FLP-GFP

Synapsin promoter dependent expression of FLP recombinase fused to GFP-P2A sequence

-

-

BL-0554

f(CMV)iCreRFP-P2A-MSCw

CMV

iCre-RFP

CMV promoter dependent expression of Cre recombinase fused to a RFP-P2A sequence

-

-

BL-0555

f(CMV)iCreGFP-P2A-MSCw

CMV

iCre-GFP

CMV promoter dependent expression of Cre recombinase fused to a GFP-P2A sequence

-

-

BL-0556

f(CMV)iCreCFP-P2A-MSCw

CMV

iCre-CFP

CMV promoter dependent expression of Cre recombinase fused to a CFP-P2A sequence

-

-

BL-0558

f(GFAP2.2)iCreRFP-P2A-MSCw

GFAP2.2

iCre-RFP

GFAP (2.2) promoter dependent expression of Cre recombinase fused to a RFP-P2A sequence

-

-

BL-0596

f(ubi)iCreRFP-P2A-MSCw

Ubiquitin

iCre-RFP

hUbiquitin promoter dependent expression of Cre recombinase fused to a RFP-P2A sequence

-

-

BL-0597

f(ubi)iCreGFP-P2A-MSCw

Ubiquitin

iCre-GFP

hUbiquitin promoter dependent expression of Cre recombinase fused to a GFP-P2A sequence

-

-

BL-0598

f(ubi)iCreCFP-P2A-MSCw

Ubiquitin

iCre-CFP

hUbiquitin promoter dependent expression of Cre recombinase fused to a CFP-P2A sequence

-

-

BL-1644

f(CAG)iCre-RFP-P2A.w

CAG

iCre-RFP

CAG promoter dependent expression of Cre recombinase fused to a RFP-P2A sequence

-

-

BL-0155

f(syn)iCreRFP-P2A-SypGFP

hSynapsin1

iCre-RFP, Synaptophysin-GFP

iCre-RFP and Synaptophysin-GFP expression coupled via P2A

-

-

BL-0290

f(syn)iCreRFP-P2A-SypmKate2

hSynapsin1

iCre-RFP, Synaptophysin-mkate2

iCre-RFP and Synaptophysin-mkate2 expression coupled via P2A

-

-

BL-0291

f(syn)iCreCFP-P2A-Sypmkate2

hSynapsin1

iCre-CFP, Synaptophysin-mkate2

iCre-CFP and Synaptophysin-mkate2 expression coupled via P2A

-

-

BL-0408

f(syn)iCre-P2A-SypGFP

hSynapsin1

iCre, Synaptophysin-GFP

iCre and Synaptophysin-GFP expression coupled via P2A

-

-

BL-1570

f(syn)iCre.P2A-EGFP WPRE

hSynapsin1

iCre, eGFP

iCre and eGFP expression under control of the human Synapsin1 promoter.

-

-

BL-1571

f(CAG)iCre.P2A-EGFP WPRE

CAG

iCre, eGFP

iCre and eGFP expression under control of the hybrid CAG promoter.

-

-

BL-1532

f(syn)Cre-P2A-PSD95-mCherry

hSynapsin1

iCre, PSD95-mCherry

iCre and PSD95-mCherry expression coupled via P2A

-

-

BL-1508

f(syn)Cre-P2A-Syp-mCherry

hSynapsin1

iCre, Syp-mCherry

iCre and Synaptophysin-mCherry expression coupled via P2A

-

-

BL-0250

f(syn)SWITCH(NLS.GFP-P2A-MCS_revRFP)

hSynapsin1

NLS.GFP, RFP

Cre-ON/OFF Vector: Expression of nuclear localized GFP (fused to P2A) in non-Cre expressing cells, Expression of RFP in Cre rexpressing cells.

-

Chang et al. 2014

BL-0251

f(syn)SWITCH(RFP_revNLS.GFP-P2A-MCS)

hSynapsin1

NLS.GFP, RFP

Cre-ON/OFF Vector: Expression of RFP in non-Cre expressing cells, Expression of nuclear GFP in Cre rexpressing cells.

-

Chang et al. 2014

BL-0261

f(syn)CreOFF(NLS.GFP-P2A-MCS)

hSynapsin1

NLS.GFP

Expression of nuclear localized GFP in non-Cre expressing cells (GFP is fused to a P2A sequence)

-

Chang et al. 2014

BL-0315

f(syn)CreOFF(NLS.RFP-P2A-MCS)

hSynapsin1

NLS.RFP

Expression of nuclear localized RFP in non-Cre expressing cells (GFP is fused to a P2A sequence)

-

Chang et al. 2014

BL-0320

f(syn)CreON(NLS.GFP-P2A-MCS)

hSynapsin1

NLS.GFP

Expression of nuclear localized GFP (fused to P2A) in Cre expressing cells

-

Chang et al. 2014

BL-0350

f(syn)CreON(NLS.RFP-P2A-MCS)

hSynapsin1

NLS.RFP

Expression of nuclear localized RFP (fused to P2A) in Cre expressing cells

-

Chang et al. 2014

BL-851

TetO-FUW-AsclI-Puro

TetO

AsclI

Expresses AsclI and puromycin resistent gene under control of TetON promoter.

Addgene #97329

Yang et al. 2017

BL-1308

TetO-FUW-Sox2-IRES-Hygro

TetO

Sox2

Expresses Sox2 and Hygromycin resistent gene under control of TetON promoter. Sublcone of Addgene #20326

Addgene #20326

Brambrink et al. 2008

BL-1287

TetO-FUW-Dlx2-IRES-Hygro

TetO

Dlx2

Expresses Dlx2 and Hygromycin resistent gene under control of TetON promoter.

Addgene #97330

Yang et al. 2017

BL-1525

TetO-FUW-Hygro

TetO

Hygromycin

Expresses Hygromycin resistent gene under control of TetON promoter.

-

-

BL-1587

TetO-FUW-Dlx2-IRES-Blast

TetO

Dlx2

Expresses Dlx2 and Blasticidin S deaminase of Aspergillus terreus (BSD) under control of TetON promoter. Subclone from BL-1287.

Addgene #97330

Yang et al. 2017

BL-1600

TetO-FUW-DLX2.P2A-AsclI-IRES.Puro

TetO

Dlx2, AsclI

Expresses Dlx2, AsclI and puromycin resistent gene under control of TetON promoter. Subclone from Addgene #97329 and #97330

Addgene #97329 and Addgene #97330

Yang et al. 2017

BL-1812

FU-tetO-ASCL1-LMX1b-NURR1-PuroR

TetO

ASCL1, LMX1B, NURR1

Expresses AsclI, LMX1b, NURR1 (ALN TFs) and puromycin resistent gene under control of TetON promoter. Addgene #182298

Addgene #182298

Powell et al. 2021

BL-1300

U6-sgRNA-empty-TRE-HifiCas9(VP12)-P2A-hygro

U6 promoter, TetO

Cas9

High-fidelty Cas9 (VP12) expression under control of TetOn Promoter, empty U6 sgRNA expression cassette and hygromycin resistent gene, Subclone of Addgene #72247.

Addgene #72247

Kleinstiver et al. 2016

BL-1269

U6-sgRNA-empty-TRE-HifiCas9(VP12)

U6 promoter, TetO

Cas9

High-fidelty Cas9 (VP12) expression under control of TetOn Promoter and with empty U6 sgRNA expression cassette, Subclone of Addgene #72247.

Addgene #72247

Kleinstiver et al. 2016

BL-1255

pLenti-CMV-GFP-2A-oG-WPRE

CMV

Rabies Gylcoprotein oG, GFP

addgene 102984

Addgene #102984

Ciabatti al. 2017

BL-1256

pLenti-CMV-puro-2A-TEVp

CMV

TEV-protease, Puromycin

addgene 99610

Addgene #99610

Ciabatti al. 2017

BL-1722

f(CAG)VSV.G-IRES-NLS-GFP-P2A-Puro-w

CAG

VSV Glycoprotein, Puromycin

Expression of VSV-G. NLS-GFP and Puromycin

-

-

BL-1724

f(CAG)VSV.G-IRES-NLS-GFP-w

CAG

VSV Glycoprotein,

Expression of VSV-G and NLS-GFP

-

-

BL-1846

f(CAG)VSV.G-IRES-CFP3-w

CAG

VSV Glycoprotein,

Expression of VSV-G and CFP3

-

-