Katalog der Lentiviralen Vektoren

Hier finden Sie eine Übersicht zu unseren verschiedenen Lentiviralen Plasmid-Vektoren. Sie können in unserem Bestellsystem die entsprechende Katalognummer eintragen und ihren gewünschten Virus produzieren lassen.

Externe Nutzer müssen einen MTA zwischen der Charité und ihrer Einrichtung abschließen. Bei einigen Vektoren müssen entsprechende Addgene Regularien angewendet werden. Nähere Informationen erhalten Sie hierzu während des Bestellprozesses.

 

 

 

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Lentivirale Werkzeuge für Ihre Wissenschaft

Mit welchem Vektor können wir Ihnen helfen? Sortiert nach ihren spezifischen Transgenen, finden Sie hier verschiedenste Werkzeuge, die Ihnen in Ihrer wissenschaftlichen Fragestellung helfen könnte.

Wir versuchen die Liste immer aktuell zu halten. Kontaktieren sie uns bitte falls ihr gewünschter Vektor nicht dabei ist. Nutzen Sie unseren DNA Service falls wir vorhandene Vektoren für Sie spezifisch modifizieren könnten.

Optogenetische Sensoren für Neurowissenschaften

Catalog # Vector name Promoter Discription MTA informations Ref for publication
HL-0121 f(syn)-VGlut1pH-w hSynapsin1 VGlut1 fused to pHLuorin in within the luminal part of the Transporter, subclone of addgene #24478 Addgene #24478 Granseth et al. 2006 and Vogelmaier et al. 2006
BL-0247 f(syn)-VGlut2pH-w hSynapsin1 VGlut2 fused to pHLuorin in within the luminal part of the Transporter, subclone of addgene #24478 Addgene #24478 Granseth et al. 2006 and Herman et al. 2014
BL-0336 f(syn)-Phönix-w hSynapsin1 Halorhodopsin fused to SypHy and mkate2 (synaptic vesicle proton pump and pH sensor) Addgene #70111 Rost et al. 2016
BL-0344 f(syn)-Syt1-pHluorin-w hSynapsin1 Synaptotagmin1 fused N terminally with pHLuorin, subclone of addgene #24478 Addgene #24478 -
BL-0047 f(syn)-SypHy-w hSynapsin1 Expression of Rat Synaptophysin-pHluorin, subclone of addgene #24478 Addgene #24478 Granseth et al. 2006
BL-0553 f(syn)-Syp-pH-mkate2-w hSynapsin1 Synaptophysin with Luminal pHLuorin and cytosolic mkate2 - -
BL-0570 f(syn)-NLS-GFP-P2A-GCamp6f-w hSynapsin1 Nuclear GFP linked via P2A to GCamp6f (Subclone of addgene #40755) Addgene #40755 -
BL-0592 f(syn)-post-mGRASP-2A-dTomato-w hSynapsin1 For mapping synaptic connectivity: postsynaptic mGRASP linked via 2A to dTomato (subclone from addgene clone #34912) Addgene #34912 Kim et al. 2011
BL-0593 f(syn)-pre-mGRASP-mCerulean-2A-nls-mCherry-w hSynapsin1 For mapping synaptic connectivity: presynaptic mGRASP fused to mCerulean and linked via 2A to NLS-mcherry (subclone from addgene clone #34911) Addgene #34911 Kim et al. 2011
BL-0638 f(syn)-NLS-RFP-P2A-post-mGRASP-w hSynapsin1 For mapping synaptic connectivity: Nuclear RFP linked via P2A with postsynaptic mGRASP (subclone from addgene clone #34912) Addgene #34912 Kim et al. 2011
BL-0639 f(syn)-NLS-GFP-P2A-pre-mGRASP-mkate2-w hSynapsin1 For mapping synaptic connectivity: Nuclear GFP linked via P2A with presynaptic mGRASP fused to mkate2 (subclone from addgene clone #34911) Addgene #34911 Kim et al. 2011
BL-0647 f(syn)SWITCH(RFP_revSypHy)w hSynapsin1 CreON/OFF Vector: Expression of RFP in Non-Cre cells, and of Rat Synaptophysin-pHluorin in Cre cells. Subclone of addgene #24478 Addgene #24478 Granseth et al. 2006
BL-0664 f(syn)CreON(SypHy)-w hSynapsin1 CreON Vector: Expression of Rat Synaptophysin-pHluorin in Cre cells. Subclone of addgene #24478 Addgene #24478 Granseth et al. 2006
BL-0676 f(syn)CreOFF(SypHy)-w hSynapsin1 CreOFF Vector: Expression of Rat Synaptophysin-pHluorin in Non-Cre cells. Subclone of addgene #24478 Addgene #24478 Granseth et al. 2006
BL-0700 f(syn)-Syp-GCamp6f-w hSynapsin1 Synaptophysin fused C terminally with GCamp6f (subclone of addgene #40755) Addgene #40755 Chen et al. 2013
BL-0987 f(syn)-NLS-RFP-P2A-SEP-GluR1 hSynapsin1 Expression of nuclear RFP linked via P2A to GluR1 fused to super-ecliptic pHlourin, subclone of addgene# 24000 Addgene #24000 Kopec et al. 2006
BL-0988 f(syn)-NLS-RFP-P2A-SEP-GluR2 hSynapsin1 Expression of nuclear RFP linked via P2A to GluR2 fused to super-ecliptic pHlourin, subclone of addgene# 24001 Addgene #24001 Kopec et al. 2006

Optogenetische Schalter für Neurowissenschaften

Catalog # Vector name Promoter Discription MTA informations Ref for publication
BL-0336 f(syn)-Phönix-w hSynapsin1 Halorhodopsin fused to SypHy and mkate2 (synaptic vesicle proton pump and pH sensor) Addgene #70111 Rost et al. 2016
BL-0347 f(syn)-ChR2-E123T/T159C-YFP-w hSynapsin1 ChR2 (ChetaTC) fused to YFP. Subclone of addgene #35511 Addgene #35511 Bernd et al. 2011 and Mattis et al. 2011
BL-0365 f(syn)-CreON(ChR2-E123T/T159C-YFP)-w hSynapsin1 CreON Vector: ChR2 (ChetaTC) fused to YFP. Subclone of addgene #35511 Addgene #35511 Bernd et al. 2011 and Mattis et al. 2011
BL-0370 f(syn)-SWITCH(RFP_revChR2-E123T/T159C-YFP)-w hSynapsin1 CreON/OFF Vector: Expression of RFP in Non-Cre cells, Expression of ChR2 (ChetaTC) in Cre cells. Subclone of addgene #35511 Addgene #35511 Bernd et al. 2011 and Mattis et al. 2011
BL-0644 f(syn)-CreON(NLS-RFP-P2A-ChR2-ET/TC)-w hSynapsin1 CreOn Vector: NLS-RFP linked to fast ChR2 (ChetaTC) via P2A, Cre dependent expression, subclone of addgene #35511 Addgene #35511 Bernd et al. 2011 and Mattis et al. 2011
BL-0662 f(syn)-CreOFF(ChR2-E123T/T159C-YFP)-w hSynapsin1 CreOFF Vector: Expression of ChR2 (ChetaTC) in Non-Cre cells. Subclone of addgene #35511 Addgene #35511 Bernd et al. 2011 and Mattis et al. 2011
BL-0679 f(syn)-ChR2-H134R-YFP-w hSynapsin1 Expression of Chr2-H134R fused to YFP, subclone of addgene #26973 Addgene #26973 -
BL-0693 f(syn)-ChR2-E123T/T159C-YFP-P2A-MCS-w hSynapsin1 ChR2 (ChetaTC) fused to YFP and linked via a P2A sequence to a multiple cloning site (MSC). Subclone of addgene #35511 Addgene #35511 Bernd et al. 2011 and Mattis et al. 2011
BL-0792 f(syn)-VChR1-eYFP-w hSynapsin1 Expression of Red-shifted volvox ChR1 fused to YFP, subclone of addgene #20955 Addgene #20955 Zhang et al. 2008
BL-0903 f(CamKII)-ChrimsonR-GFP-w CamKII Expression of ChrimsonR fused to GFP Addgene #59049 Klapoetke et al. 2014

Optogenetische Werkzeuge für Interaktionsstudien

Catalog # Vector name Promoter Discription MTA informations Ref for publication
BL-0735 f(syn)-mCherry-Cry2PHR-w hSynapsin1 Expression of mCherry tagged Cryptochrome2, subclone from addgene #28244 Addgene #28244 Kennedy et al. 2010
BL-0726 f(syn)-CIBN-GFP-TM-Stx1a-w hSynapsin1 Expression of a truncated version of a basic helix-loop-helix protein Arabidopsis CIB1 fused to GFP and the transmembrane domain of Syntaxin1a, subclone of addgene #28246 Addgene #28246 Kennedy et al. 2010
BL-0732 f(syn)-CIBN-TM-Stx1a-w hSynapsin1 Expression of a truncated version of a basic helix-loop-helix protein Arabidopsis CIB1 fused to the transmembrane domain of Syntaxin1a, subclone of addgene #28246 Addgene #28246 Kennedy et al. 2010
BL-0802 f(syn)-mCherry-Cry2PHR-P2A-MCS-w hSynapsin1 Expression of mCherry tagged Cryptochrome2 linked to a MCS via P2A, subclone from addgene #28244 Addgene #28244 Kennedy et al. 2010
BL-1142 f(syn)nMagHigh-eGFP-CAAX hSynapsin1 Blue light dependent photoswitching system: membrane bound nMag-eGFP. Subclone of addgene #67300 Addgene #67300 Kawano et al. 2015
BL-1136 f(syn)pMagFast2(3x)-iRPF hSynapsin1 Blue light dependent photoswitching system: soluble pMag-iRFP. Subclone of addgene #67297 Addgene #67297 Kawano et al. 2015

Expression von intrazellulären Markern

Catalog # Vector name Promoter Discription MTA informations Ref for publication
BL-0443 f(syn)mkate2-P2A-GFP-ER hSynapsin1 Far red mkate2 linked via P2A to endosomal reticulum bound GFP    
BL-0452 f(syn)eGFP-ER hSynapsin1 Endosomal reticulum bound GFP    
BL-0500 f(syn)Gap-GFP-P2A hSynapsin1 Membran bound GFP due to N-terminal fused dual palmitoylation sequence of GAP43    
BL-0515 f(syn)tdtomato-P2A-GFP-ER hSynapsin1 tdtomato linked via P2A to endosomal reticulum bound GFP    
BL-0569 f(syn)Tom20-GFP hSynapsin1 Mitochondrial Tom20 fused to GFP    
BL-0625 f(syn)Rab5a-GFP-w hSynapsin1 Mouse Rab5a fused to GFP    
BL-0721 f(syn)w-PA-GFP-Actin hSynapsin1 Photoactivatable GFP variant fused to Actin, subclone from addgene plasmid 57120 Addgene #57210 Olenych et al. 2007
BL-0722 f(syn)Lifeact-mEOS3.2 hSynapsin1 Lifeact-mEOS3.2 expression, subclone from addgene plasmid 54696 into LV vector. Addgene #54696  
BL-0725 f(syn)w iRFP-PH hSynapsin1 PH domain of human PLCdelta1 (against PIP4,5) fused to iRFP MTAs für Addgene #36075 und Addgene #54786  
BL-0758 f(syn)w-eGFP-2FYFE hSynapsin1 two FYFE domains fused to GFP (PI3P binder) Addgene #36096 Burd et al. 1998
BL-0855 f(syn)LifeAct-GFP hSynapsin1 Lifeact-GFP expression, subclone from addgene plasmid 54696 into LV vector. Addgene #54696  
BL-1250 f(syn)LifeAct-mkate2 hSynapsin1 Lifeact sequence fused to mkate2, subclone from addgene plasmid 54696 into LV vector. Addgene #54696  

Markierung von Synapsen

Catalog # Vector name Promoter Discription MTA informations Ref for publication
BL-0141 f(syn)-NLS-GFP-P2A-VGlut1mkate2 hSynapsin1 Nuclear GFP linked via P2A to VGlut1 fused to mkate2 - -
BL-0177 f(syn)-Syp-GFP-w hSynapsin1 Synaptophysin fused to GFP - Sampathkumar et al. 2016
BL-0195 f(syn)-Syp-GFP-P2A-MCS-w hSynapsin1 Synaptophysin fused to GFP and a P2A Sequence - Sampathkumar et al. 2016
BL-0258 f(syn)-NLS-GFP-P2A-Syp-mkate2 hSynapsin1 Nuclear GFP linked via P2A to Synaptophysin fused to mkate2 - Sampathkumar et al. 2016
BL-0373 f(syn)-Syp-mkate2-w hSynapsin1 Synaptophysin fused to mkate2 - Sampathkumar et al. 2016
BL-0418 f(syn)-VGlut1-mkate2-w hSynapsin1 VGlut1 fused to mkate2 - -
BL-0492 f(syn)-VGlu1-GFP-w hSynapsin1 VGlut1 fused to GFP - -
BL-0760 f(syn)-NLS-RFP-P2A-Syp-CFP-T2A-MCS hSynapsin1 Nuclear RFP linked via P2A to Synaptophysin fused to CFP and linked to a second 2A site with a Multiple Cloning Site - -
BL-0856 f(syn)-LifeAct-GFP-P2A-Syp-mkate2 hSynapsin1 Expression of Lifeact-GFP linked via P2A to Synaptophysin fused to mkate2, subclone from addgene #54696 Modifiction of Addgene #54696 -
BL-1033 f(syn)-Homer1-GFP-w hSynapsin1 Expression of postsynaptic Homer fused to GFP, subclone of addgene #57461 Addgene #57461 -
BL-1034 f(syn)-Homer1-tdtomato hSynapsin1 Expression of postsynaptic Homer fused to tdtomato, subclone of addgene #57461 Addgene #57461 -
BL-1035 f(syn)-Homer1-mEOS3.2-w hSynapsin1 Expression of postsynaptic Homer fused to mEOS3.2, , subclone of addgene #57461 Addgene #57461 -
BL-1038 f(syn)-GluR1-mEOS3.2 hSynapsin1 Expression of postsynaptic GluR1 labeled with extracellulär mEOS3.2, subclone of addgene #57461 Addgene #57461 -
BL-1054 f(syn)-GluR2-mEOS3.2 hSynapsin1 Expression of postsynaptic GluR2 labeled with extracellulär mEOS3.2, subclone of addgene #57461 Addgene #57461 -
BL-1122 f(syn)-Homer1-CFP-w hSynapsin1 Expression of postsynaptic Homer fused to CFP, , subclone of addgene #57461 Addgene #57461 -

Neuronale, generelle fluoreszierende Cre abhängigen Expressionsvektoren

Catalog # Vector name Promoter Discription MTA informations Ref for publication
BL-0250 f(syn)SWITCH(NLS-GFP-P2A-MCS_revRFP) hSynapsin1 Cre-ON/OFF Vector: Expression of nuclear localized GFP (fused to P2A) in non-Cre expressing cells, Expression of RFP in Cre rexpressing cells. - Chang et al. 2014
BL-0251 f(syn)SWITCH(RFP_revNLS-GFP-P2A-MCS) hSynapsin1 Cre-ON/OFF Vector: Expression of RFP in non-Cre expressing cells, Expression of nuclear GFP in Cre rexpressing cells. - Chang et al. 2014
BL-0261 f(syn)CreOFF(NLS-GFP-P2A-MCS) hSynapsin1 Expression of nuclear localized GFP in non-Cre expressing cells (GFP is fused to a P2A sequence) - Chang et al. 2014
BL-0315 f(syn)CreOFF(NLS-RFP-P2A-MCS) hSynapsin1 Expression of nuclear localized RFP in non-Cre expressing cells (GFP is fused to a P2A sequence) - Chang et al. 2014
BL-0320 f(syn)CreON(NLS-GFP-P2A) hSynapsin1 Expression of nuclear localized GFP (fused to P2A) in Cre expressing cells - Chang et al. 2014
BL-0350 f(syn)CreON(NLS-RFP-P2A) hSynapsin1 Expression of nuclear localized RFP (fused to P2A) in Cre expressing cells - Chang et al. 2014

Rekombinase Expressionsvektoren

Catalog # Vector name Promoter Discription MTA informations Ref for publication
BL-0150 f(syn)iCreRFP-P2A-MSCw hSynapsin1 Codon improved Cre recombinase fused to a RFP-P2A sequence - Vardar et al. 2016
BL-0224 f(syn)iCreCFP-P2A-MSCw hSynapsin1 Codon improved Cre recombinase fused to a CFP-P2A sequence - -
BL-0300 f(syn)iCre-P2A-MSCw hSynapsin1 Codon improved Cre recombinase fused to a P2A sequence - -
BL-0366 f(syn)iCreGFP-P2A-MSCw hSynapsin1 Codon improved Cre recombinase fused to a GFP-P2A sequence - -
BL-0445 f(syn)FLP-GFP-P2A-MSCw hSynapsin1 FLP recombinase fused to GFP-P2A sequence - -
BL-0554 f(CMV)iCreRFP-P2A-MSCw CMV Codon improved Cre recombinase fused to a RFP-P2A sequence - -
BL-0555 f(CMV)iCreGFP-P2A-MSCw CMV Codon improved Cre recombinase fused to a GFP-P2A sequence - -
BL-0556 f(CMV)iCreCFP-P2A-MSCw CMV Codon improved Cre recombinase fused to a CFP-P2A sequence - -
BL-0558 f(GFAP)iCreRFP-P2A-MSCw GFAP Codon improved Cre recombinase fused to a RFP-P2A sequence - -
BL-0596 f(ubi)iCreRFP-P2A-MSCw Ubiquitin Codon improved Cre recombinase fused to a RFP-P2A sequence - -
BL-0597 f(ubi)iCreGFP-P2A-MSCw Ubiquitin Codon improved Cre recombinase fused to a GFP-P2A sequence - -
BL-0598 f(ubi)iCreCFP-P2A-MSCw Ubiquitin Codon improved Cre recombinase fused to a CFP-P2A sequence - -

Cre Expressionsvektoren in Kombination mit neuronalen Markern

Catalog # Vector name Promoter Discription MTA informations Ref for publication
BL-0155 f(syn)iCreRFP-P2A-SypGFP hSynapsin1 iCre-RFP and Synaptophysin-GFP expression coupled via P2A - -
BL-0290 f(syn)iCreRFP-P2A-SypmKate2 hSynapsin1 iCre-RFP and Synaptophysin-mkate2 expression coupled via P2A - -
BL-0291 f(syn)iCreCFP-P2A-Sypmkate2 hSynapsin1 iCre-CFP and Synaptophysin-mkate2 expression coupled via P2A - -
BL-0408 f(syn)iCre-P2A-SypGFP hSynapsin1 iCre and Synaptophysin-GFP expression coupled via P2A - -

Generelle fluoreszierende Expressions Vektoren

Catalog # Vector name Promoter Discription MTA informations Ref for publication
BL-0100 f(syn)-GFP-P2A-MSCw hSynapsin1 cytosolic GFP expression restricted to neurons (fused to a P2A sequence) - -
BL-0110 f(syn)-mkate2-P2A-MSCw hSynapsin1 mkate2 expression restricted to neurons (fused to a P2A sequence) - -
BL-0140 f(syn)-NLS-GFP-P2A-MSCw hSynapsin1 nulcear localized GFP expression restricted to neurons (fused to a P2A sequence) - -
BL-0181 f(syn)-NLS-RFP-P2A-MSCw hSynapsin1 nulcear localized RFP expression restricted to neurons (fused to a P2A sequence) - -
BL-0512 f(syn)-tdTomato-P2A-MSC hSynapsin1 tdtomato expression restricted to neurons (fused to a P2A sequence) - -
BL-0526 f(CMV)-RFPw CMV Cytosolic RFP expression - -
BL-0533 f(CMV)-GFPw CMV Cytosolic GFP expression - -
BL-0534 f(CMV)-NLS-GFP-P2A-MSCw CMV nuclear localized GFP expression (fused to a P2A sequence) - -
BL-0535 f(CMV)-NLS-RFP-P2A-MSCw CMV nuclear localized RFP expression (fused to a P2A sequence) - -
BL-0540 f(GFAP)-GFP-MSCw GFAP cytosolic GFP expression restricted in astrocytes (fused to a P2A sequence) - -
BL-0541 f(GFAP)-NLS-GFP-P2A-MSCw GFAP nuclear localized GFP expression restricted to astrocytes (fused to a P2A sequence) - -
BL-0542 f(GFAP)-NLS-RFP-P2A-MSCw GFAP nuclear localized RFP expression restricted to astrocytes (fused to a P2A sequence) - -
BL-0543 f(ubi)-NLS-GFP-P2A-MSCw Ubiquitin nuclear localized GFP expression (fused to a P2A sequence) - -
BL-0544 f(ubi)-NLS-RFP-P2A-MSCw Ubiquitin nuclear localized RFP expression (fused to a P2A sequence) - -
BL-0752 f(syn)-CFP-P2A-MSC hSynapsin1 Cerulean expression (fused to a P2A sequence) - -
BL-1109 f(CAG)-GFPw CAG CAG Promoter for cytosolic GFP expression - -
BL-1161 f(CAG)-NLS-GFPw CAG CAG Promoter for nucklear localized GFP expression - -
BL-1181 f(syn)-NLS-CFP-P2A-w hSynapsin1 nulcear localized CFP expression restricted to neurons (fused to a P2A sequence) - -