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Katalog der RABV and SFV Vektoren

Hier finden Sie eine Übersicht zu unseren verschiedenen RABV und SFV Vektoren und bereits vorhandenen Viren (Ready-made). Sie können in unserem Bestellsystem die entsprechende Katalognummer eintragen und ihren gewünschten Virus produzieren lassen.

Externe Nutzer müssen einen MTA zwischen der Charité und ihrer Einrichtung abschließen und bei bei einigen Vektoren müssen entsprechende Addgene Regularien angewendet werden. Nähere Informationen erhalten Sie hierzu während des Bestellprozesses.

 

 

Sie befinden sich hier:

Rabies Virus (RABV)

An dieser Stelle finden Sie die verschiedenen angebotenen Rabies Viren. Ready-Made Rabies Viren sind alle EnvA pseudotypisiert und per Ultracentrifigation aufkonzentriert.

Catalog #

Vector name

Promoter

Discription

ready-made RABV

MTA informations

Ref for publication

BRABV-001

pSADB19dG-mCherry

RABV-B19

cytosolic mCherry expression

yes

-

-

BRABV-002

pSADB19dG-GFP

RABV-B19

cytosolic GFP expression

yes

-

-

BRABV-007

pSADB19dG-iCre-RFP

RABV-B19

Codon improved Cre recombinase fused to RFP

-

-

-

BRABV-008

pSADB19dG-ChR2-ET/TC-YFP

RABV-B19

Expression of ChR2 (ChetaTC) fused to YFP, Subclone of addgene #35511

-

Addgene #35511

Bernd et al. 2011 and Mattis et al. 2011

BRABV-009

pSADB19dG-GCaMP6

RABV-B19

Expression of GCaMP6f

-

Addgene #40755

Chen et al. 2013

AAV Vektoren essential für den Einsatz von Rabies Viren

Catalog #

Vector name

Promoter

Gene of Interest

Discription

MTA informations

Ref for publication

BA-064

pAAV-EF1a-DIO-HTB

EF1alpha

histion-GFP, TVA-R, B19G

Cre dependent expression of Histone tagged GFP linked via T2A to TVA receptor linked via E2A to Rabies B19G

Addgene #44187

-

BA-068

pAAV-Ef1a-DIO-H2B-GFP-2A-oG-WPRE-hGG

EF1alpha

histion-GFP, oB19G

Cre dependent expression of Histone tagged GFP linked via T2A to optimized Rabies B19G (oG)

Addgene #74289 and Addgene #44187

Kim et al. 2016

BA-091

pAAV-Syn-H2B-GFP-TVA-oG-WPRE3

hSynapsin1

histion-GFP, TVA, oB19G

Enhanced Expression of Histone tagged GFP linked via T2A to TVA receptor linked via E2A to Rabies B19oG

Addgene #74289 and Addgene #44187

Kim et al. 2016

BA-096

pAAV-Syn-flox(nGToG)-WPRE3

hSynapsin1

NLS-GFP, TVA, oG

Enhanced Cre dependent expression of nuclear localized (NLS) GFP linked via T2A to TVA receptor linked via E2A to Rabies B19oG

Addgene #74289 and Addgene #44187

Kim et al. 2016 and Zolnik et al. 2020

BA-155

pAAV-tetO-GFP-TVA-oG-WPRE3

TetO

GFP, TVA, oG

Expression of GFP linked via T2A to TVA receptor linked via E2A to Rabies B19oG under controll of the tetO Promoter (tetracycline transactivator (tTA) dependent expression), subclone of addgene #44187 and 74289

Addgene #44187 and Addgene #74289

Kim et al. 2016

BA-171

pAAV-tetO-flox(GFP-TVA-oG)-WPRE3

TetO

GFP, TVA, oG

Cre dependent Expression of GFP linked via T2A to TVA receptor linked via E2A to Rabies B19oG under controll of the tetO Promoter (tetracycline transactivator (tTA) dependent expression), subclone of addgene #44187 and 74289

Addgene #44187 and Addgene #74289

Kim et al. 2016

BA-188

pAAV-Syn-fDIO(nGToG)-WPRE3

hSynapsin1

GFP, TVA, oG

FLPe dependent expression of nuclear localized (NLS) GFP linked via T2A to TVA receptor linked via E2A to Rabies B19oG, subclone of addgene #44187 and 74289

Addgene #44187 and Addgene #74289

Kim et al. 2016

BA-196

pAAV-Syn-flox(Ce3ToG)-WPRE3

hSynapsin1

CFP3, TVA, oG

Cre dependent Expression of Cerulean3 linked via T2A to TVA receptor linked via E2A to Rabies B19oG under controll of the Synapsin promoter, subclone of addgene #44187 and 74289

Addgene #44187 and Addgene #74289

Kim et al. 2016

             

AAV ready made Rabies Helferviren

Catalog #

Vector name

Promoter

Gene of Interest

Discription

Serotype

MTA informations

Ref for publication

BA-64 AAV2/9

pAAV-EF1a-DIO-HTB

EF1alpha

histion-GFP, TVA-R, B19G

Cre dependent expression of Histone tagged GFP linked via T2A to TVA receptor linked via E2A to Rabies B19G

9

Addgene #44187

-

BA-91 AAV2/1

pAAV-Syn-H2B-GFP-TVA-oG-WPRE3

hSynapsin1

histion-GFP, TVA, oB19G

Enhanced Expression of Histone tagged GFP linked via T2A to TVA receptor linked via E2A to Rabies B19oG

1

Addgene #74289 and Addgene #44187

Kim et al. 2016

BA-91 AAV2/9

pAAV-Syn-H2B-GFP-TVA-oG-WPRE3

hSynapsin1

histion-GFP, TVA, oB19G

Enhanced Expression of Histone tagged GFP linked via T2A to TVA receptor linked via E2A to Rabies B19oG

9

Addgene #74289 and Addgene #44187

Kim et al. 2016

BA-96 AAV2/1

pAAV-Syn-FLEX-nGToG-WPRE3

hSynapsin1

NLS-GFP, TVA, oG

Enhanced Cre dependent expression of nuclear localized (NLS) GFP linked via T2A to TVA receptor linked via E2A to Rabies B19oG

1

Addgene #74289 and Addgene #44187

Kim et al. 2016 and Zolnik et al. 2020

BA-96 AAV2/8

pAAV-Syn-FLEX-nGToG-WPRE3

hSynapsin1

NLS-GFP, TVA, oG

Enhanced Cre dependent expression of nuclear localized (NLS) GFP linked via T2A to TVA receptor linked via E2A to Rabies B19oG

8

Addgene #74289 and Addgene #44187

Kim et al. 2016 and Zolnik et al. 2020

BA-96 AAV2/9

pAAV-Syn-FLEX-nGToG-WPRE3

hSynapsin1

NLS-GFP, TVA, oG

Enhanced Cre dependent expression of nuclear localized (NLS) GFP linked via T2A to TVA receptor linked via E2A to Rabies B19oG

9

Addgene #74289 and Addgene #44187

Kim et al. 2016 and Zolnik et al. 2020

Semliki-Forest-Viren

Catalog #

Vector name

Promoter

Discription

MTA informations

Ref for publication

 

BSFV-003

pSFV-1-GFP

SFV

cytosolic GFP expression

-

-

 

BSFV-004

pSFV-1-ChR2-H134R-eYFP

SFV

Expression of Chr2-H134R fused to YFP, subclone of addgene #26973

Addgene #26973

-

 

BSFV-005

pSFV-1-CaMPARI

SFV

Expression of CaMPARI, a calcium-modulated photoactivatable ratiometric integrator. Subclone of addgene #60421

Addgene #60421

Fosque et al. 2015 and Zolnik et al. 2017